Analysis Of The Mechanism Of Ulva Prolifera Against High Temperature And Strong Light Stress Based On Multi-omics

Ulva prolifera is one of the green tides in China.Temperature and light intensity are important environmental factors for Ulva prolifera growth.With the upgrading of sequencing technology,different levels of omics analysis have gradually developed.Studies by groups of different levels,we can understand the genetic expression of the biological mechanisms of stress,and can find out the underlying causes different phenotypes of organisms.In order to fundamentally reveal the molecular mechanism of the effects of high temperature and strong light on Ulva prolifera,this study starts from four aspects: Firstly,through proteomic analysis of high temperature and high light stress of Ulva prolifera,the differential proteins were screened and analyzed,and the Pathway enrichment analysis of differential proteins was compared to elucidate the molecular mechanism of high temperature and strong light stress.Then,through metabolomics analysis of Ulva prolifera with high temperature and strong light stress,screening differential metabolites and performing KEGG pathway analysis to elucidate its molecular mechanism.Then,through lipidomics analysis of Ulva prolifera under high temperature and strong light stress,the effect of high temperature and strong light on canola was clarified from the level of lipid molecules.Under high temperature stress,a total of 2226 proteins were identified by proteomics,and the total number of peptides was 18,100.A total of 21 up-regulated proteins and 20 down-regulated proteins were screened.High temperature mainly affects the response of cells to stimuli,reactions to reactive oxygen species,amino acid synthesis,and carbohydrate synthesis;Metabolomics studies screened 25 significant metabolites,mainly: L-aspartic acid,succinic acid,sucrose,L-glutamic acid and other metabolites;Lipidomics identified a total of 558 lipid molecules involved in 21 subclasses.One of the lipid molecules was up-regulated and 14 lipid molecules were down-regulated.The binding protein group,metabolome and lipid group were used to study the molecular mechanism of Ulva prolifera against high temperature.High temperature mainly affects metabolic pathways such as cAMP signaling pathway,amino acid metabolism and fatty acid metabolism.Under strong light stress,21 up-regulated proteins and 41 down-regulated proteins were screened by proteomics.Strong light mainly affects fat metabolism,photosynthesis,oxidative phosphorylation and other processes.Strong light mainly affects fat metabolism,photosynthesis,oxidative phosphorylation and other processes.It is indicated that under the high temperature and strong light stress,the biological function of the Ulva prolifera has responded accordingly;A total of 27 significant metabolites were identified by metabolomics studies,mainly L-glutamic acid,D-valine,?-linolenic acid,linoleic acid,D-lyxose,and galactitol.Metabolites such as sucrose;One lipid molecule was identified by lipidomics,and one lipid molecule was up-regulated and four lipid molecules were down-regulated.Differentially expressed lipids were mainly associated with lipid membranes of photosynthetic membranes;binding protein groups,metabolomes and lipid groups were used to study the molecular mechanisms of Ulva prolifera against strong light.Strong light mainly affects the metabolic pathways such as interconversion of sugars,fatty acid synthesis,steroid synthesis,photosynthesis,pyrimidine metabolism,carbohydrate metabolism,and transport and catabolism.In summary,under high temperature and strong light stress,Ulva prolifera mainly maintains normal physiological activities by regulating the expression of genes related to algae.