In Vitro Studies On Osteogenic Differentiation Enhanced By Ethmoid Sinus Mesenchymal Stem Cells And PGS-M

Objectives The research aimed at exploring the possibility of human ethmoid sinus mesenchymal stem cells(hESMSCs)as seed cells and PGS-M as scaffolds applied in the bone tissue engineering to repair the bone defects.Methods 1.The cells cultured from ethmoid sinus muscosa in vitro were verified as the mesenchymal stem cells through clone formation assay,flow cytometry and assays to test the ability of multipotential differentiation.2.The ability of osteogenic differentiation were tested through qPCR,western blot,ALP and ARS staining,ICC and ectopic bone formation assay.The effects on the proliferation of rBMSCs were tested through CCK-8,BrdU assay.The levels of the effects of hESMSCs on the osteogenic differentiation and the paracrine function were tested through qPCR,western blot,ALP and ARS staining,ELISA assay.3.We tested the physicochemical properties of PGS-M through 1H NMR,hydrophilicity,mechanical properties and scanning electron microscope.We tested the biocompatibility of PGS-M through LDH assay,cell adherent assay,BrdU.The effects of PGS-M on the proliferation and osteogenic differentiation were tested through qPCR,ICC,ALP and ARS staining.Results 1.hESMSCs were verified as mesenchymal stem cells,its capacity to differentiate into osteoblasts were strong,and it could promote the proliferation and osteogenic differentiation of rBMSCs?2.The physicochemical and biological properties of PGS-M were better than PGS,and it could promote the proliferation and osteogenic differentiation of hADSCs.Conclusions 1.The ability of hESMSCs to differentiate into osteoblasts and paracrine were powerful,and it could be a good potential seed cells for bone tissue engineering.2.PGS-M possessed appropriate physicochemical and biological properties,and its ability to promote the hADSCs to differentiate into osteoblasts were strong,which made PGS-M a promising scaffolds for bone tissue engineering.