Jasmonic Acid And Salicylic Acid Signals Are Involved In The Regulation Of Fungi-induced Anthocyanin Biosynthesis In Arabidopsis Thaliana

Anthocyanin is a kind of water-soluble pigment widely existing in plants,which has the function of protecting plants against ultraviolet radiation,antibacterial and pathogen infection.Exogenous hormones and pathogenic bacteria or fungi can stimulate anthocyanin accumulation in plants.However,the signal transduction pathway involved in fungal induction of plant anthocyanins is not clear.In this paper,we screened the fungi that can induce anthocyanin accumulation in Arabidopsis thaliana.To determine the signal transduction pathway involved in fungi-induced anthocyanin synthesis in Arabidopsis thaliana by the addition of exogenous hormones and their corresponding signaling pathway inhibitors;Effects of exogenous hormones and their corresponding signal pathway inhibitors on anthocyanin accumulation in mutants of MBW triple complex were used to determine the role of MBW triple complex in fungi-induce anthocyanin accumulation in Arabidopsis thaliana.The main results are as follows:1.The results showed that the biosynthesis of anthocyanins in Arabidopsis thaliana could be induced by four fungi,A.capillariae,aspergillus japonicus,mycosporium and gibberella,among which A.capillariae had the most obvious effect.2.After fungal induction,the contents of anthocyanin,jasmonic acid and salicylic acid in Arabidopsis thaliana were significantly increased.Transcriptome analysis and real-time fluorescence quantitative PCR showed the genes involved in anthocyanin,jasmonic acid and salicylic acid biosynthesis related genes were significantly up-regulated expressed.3.Both the jasmonic acid biosynthesis inhibitor ibuprofen and the salicylic acid biosynthesis inhibitor 2-aminindane-2-phosphonate can inhibit the accumulation of anthocyanins induced by fungi in Arabidopsis thaliana.4.Fungal treatment,exogenous jasmonic acid and salicylic acid treatment could not induce anthocyanin accumulation in WD40,bHLH and MYB deletion-mutants,but stimulated anthocyanin accumulation in plants overexpressed MYB75.5.The above results showed that the signaling pathways of jasmonic acid and salicylic acid,as well as the MBW triple complex,were involved in the biosynthesis of anthocyanins induced by fungi in Arabidopsis thaliana.