Influence Of Urbanization On The Diversity Of Insect Symbiotic Microbes

In the present,urbanization has become one of the important social phenomena.Problems such as the population is frequently assembling from the rural to the urban,over development of urban space and environmental pollution caused by urbanization will increasingly have adverse effects on biodiversity.Insects are the most widely distributed and abundant animals in nature,which have important ecological service functions such as pollination,resource supply and biological control.Insects are an important part of urban ecosystem and are deeply influenced by human activities.Hymenoptera insects are important biological pollination and biological control resources,which can greatly save labor,promote agricultural development and play a significant role in the recovery of fragile ecological environment.Insect symbiotic microorganisms can play an important role in providing nutrients for the host,increasing insect adaptability,and protecting insects from pathogens.To explore the impact of urbanization on insect body surface and intestinal microorganism will provide microbiological data basis for understanding the maintenance mechanism of urban insect diversity and have fundamental significance for the protection of biodiversity in the process of urbanization.In Jinan urbanization gradient habitat in this study,the use of malaise trap to collect insects,separation of all the insects surface microorganisms,anatomical Hymenoptera insects bowel to obtain samples of gut microbes,using high-throughput sequencing technologies for microbial 16 s r DNA sequence,the sequence of information processing than from species,using alpha diversity,community composition analysis,analysis of the difference between the sample groups and beta diversity analysis to analyze surface microorganisms and the intestinal microflora.We found that the surface microbial diversity of urban and forest habitats was lower than that of rural habitats,and there was no significant difference in intestinal microbial diversity among the three habitats.Further analysis found that,the Proteobacteria,Actinobacteria,Cyanobacteria and Cyanobacteria of the three habitats at the phylum level were significantly different from urban habitats in rural habitats and forest habitats.There were some changes in intestinal microorganism but no statistical difference.On the level of genus,Cyanobacteria,Pantoea,Enterobacter,Undibacterium,Acinetobacter and Enterococcus have significant differences in the three habitats.There were some changes in each genus of intestinal microorganism in the three habitats,but there were no statistical differences.By analyzing the common microorganisms of body surface microorganism and intestinal microorganism in the three different habitats,it is found that the common microorganisms account for a large proportion,and there are fewer species of common microorganisms between urban and rural areas and between rural and forest areas.In summary,in this study,with the high-throughput sequencing technology,the surface microbial and intestinal microbes of insects in different habitats were studied.After analysis,the change rule of surface microbes and intestinal microflora of insects in different habitats were obtained.Moreover,it is also preliminarily speculated that the microbial diversity of the surface of the insect and the microbial diversity of the intestine may be related to the development trend of urbanization,which provides a certain experimental basis for the protection of biodiversity during the development of urbanization.