| This study set out to determine tetracycline and erythromycin resistance profiles and detect various tetracycline (tetB, tetC, tetL, tetM, tetQ and tetW) or erythromycin (ermA, ermB and ermF) resistance determinants from clinical isolates of bovine foot rot bacteria isolated from affected animals in Alberta. Using agar dilution assay, we determined that 82% (32/39) of Fusobacterium necrophorum were tetracycline resistant and 62% (24/39) were classified intermediate to erythromycin. Approximately 82% (18/22) and 91% (20/22) of Prevotella melaninogenica was sensitive to tetracycline and erythromycin respectively, For Porphyromonas levii, 88% (21/24) were sensitive to tetracycline and 100% (24/24) were erythromycin sensitive. Polymerase chain reaction amplification revealed that tetC, tetL, tetM and ermF were commonly detected in F.necrophorum, and tetQ in Pr.melaninogenica and P.levii. Special resistance determinant patterns were not observed in antibiotic resistant strains compared to sensitive isolates in all three pathogens because as high as 90% of resistance determinants were detected in both resistant and sensitive strains of bacteria. |
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