| Toxic metals such as cadmium (Cd2+) have been shown to bind to and interfere with various calcium (Ca2+) binding proteins including the regulatory protein cardiac troponin C (cTnC). Recent structural data has shown that Cd2+ binds to both EF hand Ca2+ binding loops in the regulatory N-domain of human cTnC including EF hand loop I, which normally does not bind metal. Although the data reveal two Cd2+ ions bound to the protein, the binding constants and other thermodynamic parameters are not known. Therefore, the goal of this research project is to use Isothermal Titration Calorimetry (ITC) to obtain thermodynamic parameters such as K, DeltaH, DeltaG and DeltaS of Cd2+ binding to both the full length and N-domain (amino acid residues 1-89) of HcTnC and compare the parameters to our previous data with Ca2+. Through our results we hope to shed light on potential mechanisms of cadmium toxicity. |
