| Hemicellulose isolated from hybrid poplar, and oat spelt xylan were enzymatically pretreated to release fermentable carbohydrate for the production of extracellular polysaccharide (EPS) by Klebsiella pneumoniae. Optimal production of EPS on xylose was determined under the following conditions: H&M medium buffered with 100 mM sodium phosphate to a pH of 7.3, 2% xylose at a C:N ratio of approximately 10:1, growth in a submerged vigorously aerated fermenter at 32;K. pneumoniae produces an acidic extracellular polysaccharide composed of galactose, galacturonic acid, and fucose; regardless of the substrate provided for growth. However, some differences in monomer ratios have been observed under varying conditions of growth. |
