Comparison of bioaerosol collection methods in the detection of airborne influenza virus

Detection of airborne influenza virus is needed in order to determine exposure and prevent and control infections. Few researchers have successfully detected airborne influenza virus in environmental settings with current bioaerosol samplers. Therefore, new sampling strategies should be considered to increase the likelihood of detection.;This study compared four bioaerosol samplers in collection of airborne influenza virus -- the SKC Biosampler, NIOSH Biosampler, Andersen N6 single-stage impactor containing a liquid media, and the newly developed Next Generation Inhalable Aerosol Sampler (NGIAS). Ten 30-minute laboratory trials were completed by aerosolizing active influenza virus (H1N1) in a bioaerosol chamber to compare the ability of four bioaerosol samplers to collect aerosolized virus. Samples were analyzed using RT-qPCR.;The mean total virus particles per liter of sampled air (TVP) recovered with the NGIAS was significantly less than that measured by all other samplers (p < 0.001). The TVP recovered with the SKC Biosampler (111.41) and Andersen N6 sampler (102.36) was substantially larger than that recovered with the NIOSH Biosampler (58.59), however the difference in TVP between these samplers was not statistically significant (SKC -- NIOSH p-value = 0.187 ; Andersen -- NIOSH p-value = 0.297).;Our results demonstrated that liquid based bioaerosol samplers recovered more TVP than dry collection samplers. The high flow rate sampler, the Andersen N6, did not collect more TVP, but had a lower limit of detection than other samplers. Furthermore, the SKC Biosampler collected the most TVP. Therefore, future investigators should design a liquid based personal bioaerosol sampler to maximize the likelihood of influenza virus detection.