| his work involves the molecular cloning the ;Enhanced expression of Rep protein, although not deleterious to the cell, was desirable to increase the level of this protein, which is normally present at very low levels in E. coli. By cloning the rep gene into an inducible expression vector with a strong promoter is was possible to synthesize large quantities of Rep protein. In addition, the cloned rep gene was useful in constructing an E. coli strain that completely lacked rep function. The effects of increased levels of Rep protein, or its complete absence, on DNA replication were investigated.;The studies described in this thesis involve the use of expression vectors and other molecular and genetic techniques to manipulate the ;The presence of the... |
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