| The induction of phytoalexin biosynthesis in plants in response to oligosaccharide elicitors originating from the mycelial walls of the phytopathogenic fungus Phytophthora megasperma f. sp. glycinea is being used as a model system for the study of cellular signal perception mechanisms in plants. Experiments were designed to identify and purify, from soybean cells, specific binding protein(s) (putative receptors) for a hepta-;Structure-activity studies using a number of chemically synthesized oligosaccharides established that the branched trisaccharide at the nonreducing end of the hepta-;The elicitor-binding sites appeared to be (glyco)proteins integrated in the plant plasma membrane. The elicitor-binding proteins were solubilized from membranes using the non-ionic detergent, n-dodecylsucrose. The detergent-solubilized elicitor-binding proteins retained the ligand-specificity and binding affinity (apparent K... |
