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Activation of Bacillus thuringiensis spores by alkaline pH is dependent upon protoxin-encoding plasmids

Posted on:1994-11-08Degree:M.SType:Thesis
University:Stephen F. Austin State UniversityCandidate:Newnam, Kimberly AdeleFull Text:PDF
GTID:2473390014493505Subject:Microbiology
Abstract/Summary:
The extent of spore activation was compared between protoxin crystal-forming strains of Bacillus thuringiensis subspecies kurstaki and Bacillus cereus and acrystalliferous strains of B. thuringiensis kurstaki and B. cereus. Crystal-forming strains of both species germinated better after incubation in alkaline pH than they did at neutral pH, and also their germination rates were greater than the acrystal-liferous strains incubated in alkaline pH. This indicates that the dissolution of the protoxin crystal by alkaline pH plays a key role in activation of these spores by alkaline pH.
Keywords/Search Tags:Alkaline ph, Activation, Bacillus thuringiensis, Crystal-forming strains
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