Novel beta-lactam antibiotics: Study of their mode of action against beta-lactam recognizing enzymes

Since the pioneering work of du Vigneaud in the 1940s, conventional wisdom and practice assume that compounds derived from 6-D-aminopenicillanic acid (D-APA) and 7-aminocephalosporanic acid (7-ACA) should represent the most active forms. The new short four-step synthesis of ;Two analytical methods have been developed. The first is a method developed for the separation of L-APA zwitterionic diastereomers by anion-exchange chromatography. Four L-APA diastereomers can be separated with one of them being totally resolved. Hydrophobicity plays an important factor in this separation. The second is the development of a nonradioisotopic, fluorescent/spectroscopy in combination with chromatography based techniques for identification and quantification of the PBPs targets of these novel...