| In the thesis studies, three related projects using SSNMR were carried out on proteins and peptides in solids. In the first project, we implemented high-resolution SSNMR to inspect the secondary structural formation of peptides in the course of SPPS to gain structural insight into the cause of drastically reduced synthesis yield for a long peptide sequence. In the second project, a novel SSNMR approach was established to assist the side-chain signal assignment of trace amount proteins by enhancing both sensitivity and resolution. In the third project, we established a novel SSNMR spectral editing method, termed HIGHLIGHT, to extremely simplify the SSNMR spectra under ultra-fast MAS condition. |
