| Objective: In cases of advanced bone loss, nCS graft cannot be solely used as a filler material due to, the graft fast biodegradation rate and the insufficient mechanical strength. The study goal was to assess HOB's viability and activity rates when they are cultured in media supplemented by two sources of freshly mixed nCS grafts with/out uniquely malleable Ti meshes. Methods: Primary human osteoblast HOB cells were incubated in alpha-MEM, %10 FBS and %1 antimycotics, at %100 humidified air containing %95 O2, %5 CO2, at 37 °C. There were six different conditions of the incubating media; pure media (Control), Ti mesh alone, Ti mesh with nCS or Nanogen, nCS alone or Nanogen alone. MTT and ALP assays were conducted to measure the cells growth and activity by using the scanning multiwall spectrophotometer. Using Unistat and SPSS soft wares, Two-way ANOVA was conducted and followed by Tukey HSD test. Quad two way ANOVA and a follow up t distribution tests were conducted in case of homogeneity test violation or to overcome the limitation of the small used sample size. Results: Analysis of MTT data, regardless of the nCS source, Ti mesh supplementation always enhanced the HOB's mean growth rate when compared to cultures lacking the mesh. Analysis of ALP assay data, revealed a positive significant impact of the Ti mesh on the HOB's mean activity rate. Cultures supplemented by Nanogen and Ti meshes yielded the highest HOB's mean activity rate followed by cultures supplemented by only nCS graft. Conclusion: In cases with advanced bone loss, supplementing the nCS graft material with Ti mesh will provide a strong scaffold that will guarantee fast elimination of the graft material at a closely similar pace of the new bone formation process along with a more accurate radiographic assessment. The nCS fast biodegradation will minimize any likelihood of graft-implant contact and subsequently yields better clinical outcomes. That will supposedly allow for early implant placement. |
