Differential gene expression in response to sub-zero temperatures in the freeze tolerant wood frog, Rana Sylvatica

Exposure to subzero freezing temperatures causes a variety of biochemical changes in the freeze-tolerant wood frog, Rana sylvatica. Cellular responses to freezing commence a mere five minutes after the initial formation of epidermal ice. To obtain a general overview of differential protein expression in response to freezing, in vitro translation of mRNA isolated from tissues (liver, brain, heart, muscle, kidney and gut) was conducted and compared for control (5...