| Cryopreservation of germ is going to play a critical role in sustaining the earth's threatened animal biodiversity. The first objective of this study was to examine the post-thaw effects of, three cryoprotective extenders, three freezing rates, and three thawing rates on coyote spermatozoa. After thawing, my findings established that coyote spermatozoa frozen at a moderate freezing rate (−6°C/min) in a Tris-fructose extender, and thawed at slow rate (37°C water bath for 120 seconds), resulted in more vigorous post-thaw motility (57.5%). I also examined the importance of the route of insemination when using fresh and frozen semen. My findings indicated that when using fresh semen, deposition site (vaginal 63.6%, intrauterine 63.6%) did not seem to have an effect on pregnancy rate. There was, however, a drastic decline in the number of coyotes that became pregnant when frozen semen was deposited both vaginally (0.0%) and intrauterine (18.2%). |
