| Didemnin B is a cyclic marine depsipeptide with potent cytotoxic and immunosuppressive properties. In this work the mechanism of inhibition of in vitro protein synthesis by didemnin B has been evaluated. Didemnin B is an inhibitor of eukaryotic protein synthesis. Didemnin B stimulates eEF-1;During eEF-1;We propose that didemnin B stabilizes the eEF-1;The effect of didemnin B upon other biochemical pathways that are mediated by eEF-1;We have also established the mechanism of protein synthesis inhibition by RA-VII, a naturally occurring cyclic hexapeptide. RA-VII inhibits peptidyltransferase activity of the 80S ribosome, unlike bouvardin, another cyclic hexapeptide with very close structural resemblance to RA-VII. Bouvardin inhibits eEF-2-catalyzed translocation of aminoacyl-tRNA from the A-site to the P-site on the 80S ribosome. |
