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Crystal structure and catalytic mechanism of the catalytic domain of the beta-1,4-glycanase Cex

Posted on:1998-01-31Degree:Ph.DType:Thesis
University:University of Toronto (Canada)Candidate:White, AndreFull Text:PDF
GTID:2461390014975340Subject:Chemistry
Abstract/Summary:
Cellulose-degrading microorganisms secrete a number of glycosyl hydrolases to degrade plant cell walls, the most abundant and renewable source of biomass. The bifunctional xylanase/cellulase (;This thesis reports the high resolution (1.8 A) crystal structure of cex-cd which is determined by the classical method of isomorphous replacement using heavy atoms. The main architecture of cex-cd consists of an elliptic (;The structure of cex-cd determined in the presence of 2FDNPC reveals a fluorocellobioside molecule at the carboxy-terminal end of the (...
Keywords/Search Tags:Structure
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