| Capillary electrochromatography (CEC) is an emerging electroseparation technique that is quickly gaining popularity. The synthesis and evaluation of various capillaries for separation by open tubular chromatography (OTCEC) are described. Fused silica capillaries with an i.d of 50 mum are etched and then chemically modified by the silanization/hydrosilation process to attach 1-octadecyl and/or 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) stationary phases. These capillaries are then used for separations of a number of different types of compounds such as mixtures of lysozymes, tetracycline antibiotics etc. In comparison to bare 50 mum capillaries and unetched but chemically modified 50 mum capillaries, the etched and chemically modified capillaries show better separation under identical conditions of buffer pH. Electroosmotic flow (EOF) measurements over the pH range of 2.14--7.40 for some of the chemically modified capillaries are presented. |
