Influence of silver nanoparticles on fluorescence enhancement

In this project, we studied the influence of silver nanoparticles (AgNP) on the fluorescence properties over glass substrates and on Nano pours oxide film substrates. Enhanced fluorescence intensities were observed due to presence of AgNP in near field to a fluorophore creating hot spots thereby proving the concepts of Metal Enhanced Fluorescence and Surface Plasmon Resonance (SPR). A Bragg reflector pattern is created by varying the refractive index of surface resulting in layered structure causing the light getting trapped in between the layers which exhibited the properties of SPR and Evanescent fields. The mean intensity values were higher for the AgNP introduced glass samples compared to the controls, which provided an evidence of Fluorescence Enhancement. This property may be useful for designing new analytical tools that can be used for single molecule detection in the fields of bio sensing and bio imaging.