| The goal of this research was to elucidate multiple antimicrobial activities demonstrated by the marine red alga Spyridia filamentosa (Wulfen) Harv. Preliminary separation of active principals from crude lipid-soluble extracts was achieved using thin layer chromatography (TLC). Three chromatographic zones from the TLC separation (zone 5, 9 and 12) were chosen and further analyzed by high performance liquid chromatography (HPLC). Forty-eight individual HPLC fractions from the three TLC zones were collected and assayed against five pathogenic microorganisms. Nineteen different antibiotic compounds (seven for TLC zone 5, five for TLC zone 9 and seven for TLC zone 12) inhibit growth against at least one assayed microorganism. This study demonstrates that biological activity in S.filamentosa is due to numerous metabolites, each of which possessed different activities against different microorganisms. s. |
