2,4,6-trinitrotoluene reduction by hydrogenase in Clostridium acetobutylicum | Posted on:2004-12-10 | Degree:M.S | Type:Thesis | University:Rice University | Candidate:Watrous, Mary Margaret | Full Text:PDF | GTID:2461390011976005 | Subject:Biology | Abstract/Summary: | | Unique genetic modifications to C. acetobutylicum altered the level of hydrogenase expression, allowing study of the resulting effects on the 2,4,6-trinitrotoluene (TNT) reduction rates to be possible. A strain designed to over-express the hydrogenase gene resulted in maintained TNT reduction during late growth phases when it is not typically observed. Strains exhibiting under-expression of hydrogenase produced slower TNT rates of reduction correlating to the expected inhibition of each strain type. Hydrogenase activity, measured by hydrogen production, in Clostridium acetobutylicum correlates strongly (R2 = 0.89) to TNT reduction rates. Indications suggested that hydrogenase potentially played an integral role in catalysis of TNT transformation by reducing its nitro substituents to the corresponding hydroxylamines. A mechanistic pathway is proposed by which this transformation takes place and may enhance the understanding of commonly found hydrogenases in other microorganisms and their ability to transform nitroaromatic compounds. | Keywords/Search Tags: | Hydrogenase, Reduction, TNT | | Related items |
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