| Glioblastoma multiforme (GBM) is a malignancy known for its rapid progression. During tumor invasion, the breakdown of brain tissue releases lipid-associated foreign contaminants. This study assesses the growth response of two human glioblastoma cell lines, T98G and U138-MG, to environmental chemicals stored within the brain. Proliferative or cytotoxic responses to treatment were determined using an MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] metabolic activity assay and morphological analysis. Although cytotoxic effects were seen in both T98G and U138-MG cells, proliferative responses occurred only in the T98G cell line. DDT, DDE, and PCB 153 were cytotoxic in both lines, while PCB 81 caused cell death only in T98G cells at lower concentrations. Testosterone, nonadecafluorodecanoic acid (NDFDA), chlorpyrifos, and PCB 77 stimulated the proliferation in the T98G cells. Exposure to these substances may increase the rate of glioblastoma tumor cell growth and result in the development of more aggressive forms of GBM tumors. |
