Font Size: a A A

Comparison of the PremiTest and xMAP Salmonella serotyping assays and classical serotyping for determination of Salmonella serovars

Posted on:2013-12-23Degree:M.SType:Thesis
University:Iowa State UniversityCandidate:Morningstar-Shaw, Brenda ReneeFull Text:PDF
GTID:2453390008978156Subject:Biology
Abstract/Summary:
Two commercial molecular assays, the PremiTest Salmonella and the xMAP Salmonella, were recently developed with the goal of identifying many of the most common Salmonella enterica serovars in less than eight hours. This study compared the results of these two assays to that of traditional serotyping on 233 isolates representing more than 52 different serovars, including the ten most frequently identified at the NVSL from 2000 to 2010. The PremiTest assay completely and correctly identified 150 isolates (64%) and the xMAP assay identified 181 isolates (78%). Both molecular assays were able to partially identify additional serovars. These assays offer a new, rapid approach to screening Salmonella isolates in the laboratory.
Keywords/Search Tags:Salmonella, Assays, Premitest, Xmap, Serovars, Serotyping, Isolates
Related items