| Sclerotinia stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is an economically important pathogen on canola (Brassica nanups L.). Due to the increasing public concerns of fungicide usage and the limitations of cultural methods, biological control is emerging as a promising alternative to control S. sclerotiorum in an environmentally-friendly way.; Several bacterial strains, mainly Bacillus spp., were isolated from canola and wheat plants. Eight Gram-positive bacterial strains were inhibitory against mycelial growth of S. sclerotiorum in intro . Three Bacillus spp., one Staphylococcus spp. and one Pseudomonas spp. (control) were tested in whole plant assays.; To identify antibiotic-related genes in these potential biocontrol bacterial agents, polymerase chain reaction (PCR) with specific primers was used.; Induced resistance mediated by S. sclerotiorum and B. amyloliquefaciens BS6 was evaluated in a greenhouse study followed by analysis of the phenolic compounds from canola leaf extracts by high performance liquid chromatography (HPLC). (Abstract shortened by UMI.)... |
