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Discovery and utilization of molecular markers for genetic studies of common bacterial blight resistance and seed coat colour in Phaseolus vulgaris L

Posted on:2006-11-03Degree:M.ScType:Thesis
University:University of Guelph (Canada)Candidate:Larsen, Roger James AFull Text:PDF
GTID:2453390005496929Subject:Biology
Abstract/Summary:
Common bacterial blight (CBB) is a major disease in common bean (Phaseolus vulgaris L.) caused by Xanthomonas axonopodis pv. phaseoli. Across between CBB resistant OAC Rex and SVM Taylor Hort. was made to introduce CBB resistance into cranberry beans. Populations obtained by selfing segregated for a wide variety of seedcoat colours and patterns. Testcrossing determined the genotype of OAC Rex to be [C]GBVJ and the genotype of SVM Taylor Hort. to be [ CRst] gbvJ and [CRst] gbvlaeJ. Molecular marker analyses confirmed the genotypes of the parents. Disease screening data for 157 recombinant inbred lines (at the F4 and F5 generations) from the SVM Taylor Hort x OAC Rex cross found CBB resistance associated with a growth habit gene as well as with markers on with linkage group B7. Resampling and oxford plot simulation analysis of mapped DNA sequences in bean, Arabidopsis, and soybean, found evidence for syntenic relationships.
Keywords/Search Tags:SVM taylor hort, CBB, OAC rex, Resistance
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