BPMV effects on ten experimental soybean lines and cultivars

Bean pod mottle virus (BPMV) effects were evaluated on ten experimental soybean lines and cultivars during 2002 and 2003 at two South Dakota sites. Soybeans evaluated included Ag2302, DK B28-51, SD00-1312R, SD93-828E, SD99-026R, SD99-048R, SD99-085R, SD99-096R, SD99-99R, and T-3205RR. Plants were spray-inoculated at V3-V5 with a 1:10 extract of BPMV-infected tissue macerated in 0.02 M Na and K PO4 buffer pH 7.2 with 1% silica carbide (w/v) at 552 kPa. Comparisons of BPMV-inoculated versus non-inoculated treatments indicated that all evaluated soybeans were significantly affected by BPMV (P < 0.0001). Yield losses ranged from 0.9% to 19.4% in 2002 and 16.8% to 56.6% in 2003. BPMV significantly affected test weight; number of nodes, pods, and seeds per soybean plant; number of seeds per pod; percentage of oil and protein in soybean seed; height; disease incidence; lodging; and leaf and plot ratings for mottling and distortion.