| Dengue fever is a mosquito born viral disease that is reaching epidemic proportions. Despite of various control means, it is still flourishing and is becoming a major health concern around the world. In this thesis, a novel diagnosis method for the detection of Dengue virus has been developed. Micro-spot-integrated pillars chip (MSIP) has been used to increase the surface area for the immunoreaction and hence the probability of the Dengue virus detection. The MSIP chip used for the diagnosis of Dengue NS1 was chemically modified to immobilize the capture antibodies. Each step of the surface modification and biofunctionalization process was characterized by using different characterization tools. An automated and unsupervised algorithm was developed to quantify the fluorescent signal. The result showed that the method developed was five fold more sensitive than the immunoassay performed on chemically modified at silicon substrate and hence, meet the objective of the thesis. |
