A data processing strategy for the computational identification of novel mRNA transcripts and novel mRNA splice-forms from RNA-seq data generated by massively parallel sequencing

This thesis details the development of an analytical data processing strategy to computationally identify novel mRNA transcripts and novel mRNA splice-forms. Included in this thesis will be the discussion of sequencing technologies, gene expression technologies, RNA-Seq data manipulation, the programs used to find exon-exon splice junctions in genes and downstream analysis tools and strategies.