| Osteoclasts differentiation is regulated by a complex series of genes. HDACs, histone deacetylases, are a family of proteins that inhibit gene expression in multiple cell types. Class II HDACs are expressed in multiple cell types and found either in the nucleus or the cytoplasm or both cellular compartments of cells. This localization suggests that they have roles in regulating cell differentiation. The Mansky lab and other have demonstrated that the class II HDACs, HDAC7 and HDAC9, act as inhibitors of osteoclast differentiation. The aim of my project was to investigate the role of HDAC10, another class II HDAC, in regulating osteoclast differentiation. Osteoclasts expressing a shRNA against HDAC10 were larger in size and demineralized more of a calcium/phosphate substrate compared to control infected cells. This data suggests that similar to HDAC7 and HDAC9, HDAC10 acts as an inhibitor of osteoclast differentiation. |
