Diversity And Function Of Endophytic Fungi In Zushima

Daphne giraldii Nitsche is a perennial herbaceous plant.The Chinese Pharmacopoeia records the dried stem bark and root bark of Daphne giraldii Nitsche plant of Thymelaeaceae(Thymelaeaceae).Dispelling wind and dampness,dispersing blood stasis and relieving pain,folks said:"When the pain is crawling all over the ground,use ancestral hemp." At present,the resources of ancestor hemp are over-picked,and the development of artificial cultivation is lagging,which leads to the unmet market demand.High-performance liquid chromatography and other technologies provide new directions and guidance for the rational application of ancestral hemp resources,best cultivated areas of CORTEX DAPHNES and the standardization of its modern cultivation.The high-throughput sequencing method was used to analyze the diversity of endophytic fungi in ancestors of different origins;and the traditional single colony isolation method was used to isolate and purify endophytic fungi from ancestors in different origins,and combined with morphological and molecular identification methods for endophytic fungi The identification of the strains complements the research on the diversity analysis of endophytic fungi;then screens the strains that can inhibit E.coli and Staphylococcus aureus by the Oxford Cup inhibition zone test;finally,the endophytic fungi and ancestors are detected by HPLC The two-way fermentation products of medicinal materials are used to screen strains that can increase the content of ancestral hemp A,which provides a theoretical basis for the rational and sustainable development of ancestral hemp resources.The specific research results are as follows:The topic has achieved the following research results:(1)Using Illumina MiSeq high-throughput sequencing technology to amplify and sequence ancestors of ancestors from different origins,obtaining a total of 632,766 valid sequences,and through OTU and species community analysis,Alpha Diversity analysis and other methods on ancestors of ancestors Based on the analysis of related diversity,the results of high-throughput sequencing showed that there were a total of 348 OTU endophytic fungi,4 phyla,20 classes,48 orders,108 families,154 genera,and 208 species,of which Altemaria was dominant Genus,endophytic fungi diversity analysis showed that the ancestor fungi diversity of the ancestors at the three sampling sites was Gangu County>Wutai County>Dangchang County.t(2)Using traditional single colony separation method to obtain 711 endophytic single colonies,using the combination of morphology and molecular biology,36 species of endophytic fungi can be identified.According to the diversity calculation method,different origins are analyzed The diversity of endophytic fungi species in ancestral hemp is the same as the first chapter.(3)Using the Oxford Cup bacteriostatic circle test,one endophyte fungus that can produce E.coli growth is found,and two endophyte fungi that can inhibit the growth of Staphylococcus aureus are found.(4)Using HPLC method to detect and screen one strain that can increase the content of the active ingredients of the ancestral medicinal materials,the susceptible resources of the ancestral hemp provide a new direction and guidance.