| Objective:To establish a rat model of Precancerous lesions of gastric cancer(PLGC)and to clarify the effective experience of Professor Lu Zhizheng,a master of traditional Chinese medicine,in the treatment of gastric precancerous lesions.Manpixiao treats PLGC rats by observing its pathological morphology and parietal cell ultrastructure Change the situation,combine macroscopic pathology,microstructure and network pharmacology,explore the mechanism of PLGC from the organization,cell and gene level,and provide theoretical basis and experimental support for the inheritance of academic experience of famous doctors and famous experts and the development of traditional Chinese medicine.Methods:76 male Wistar rats were randomly divided into normal group 24 and model group 52.The normal group was reared routinely,eating and drinking freely;the model group adopted four-factor combined modeling method,that is,drinking MNNG solution freely,eating Ranitidine feed freely,combined with hunger and satiety disorder and sodium salicylate solution gavage.At the 32nd week of modeling,10 rats were randomly selected from the normal group and the model group.and PLGC replication in the model group was determined according to the general conditions of the rats in each group and gastric histopathological staining sections.The remaining 42 rats in the model group were continued to be modeled as described above,and were randomly divided into a blank model group,Weifuchun group,and Manpixiao group,with 14 rats in each group.The Weifuchun group was gavaged with the Weifuchun solution at a dose of 0.432g/(kg·d);the Manpixiao group was gavaged with the Manpixiao solution at a dose of 1.45g/(kg-d);the normal group and the blank The model group was given intragastric administration of the same volume of high-temperature and high-pressure sterilized water.To make a pathological section of the stomach tissue,observe the pathological morphology of the rats in each group,and to evaluate the therapeutic effect of Manpixiao on PLGC rats;make an electron microscope section of the stomach tissue electron microscope section was prepared to observe the ultrastructure of pariolar cells under transmission electron microscope.The observation contents included secretory tubules,vesicle system,mitochondria,etc.Combined with the network pharmacology technology,the comprehensive study was conducted to explore the mechanism of PLGC by improving the ultrastructure of gastric pariocyte.Result:1.PLGC model is established1.1 The eyes,nose,ears,paws,and fur of the normal group were normal.while the eyes of the rats in the model group were dim,the ears,nose and claws were pale white and the hair was sparse..1.2 The weight of the model group was significantly different from that of the normal group(P<0.01).1.3 In the normal group,the gastric tissue structure was intact and normal,while in the model group,the gastric tissue structure was defective,with mucosa atrophy and thinning,and the number of glands decreased,accompanied by intestinal metoplasia and dysplasia.2.The curative effect of Manpixiao elimination intervention2.1 the eyes of the rats in the blank model group were dull,and the ears,nose,and paws were all pale,the paws were desquamated,and the hair was dry.which was better than the Weifuchun group.2.2 The weights of rats in the blank model group,Weifuchun group and Manpixiao group are lower than normal control group(P<0.01),there is a significant difference.2.3 The gastric tissue structure of rats in the normal control group was normal.The gastric tissue structure of the blank model group showed typical dysplasia,and the intestinal metaplasia was reduced.The gastric tissue structure of the Manpixiao group recovered close to the normal control group and was superior to the Weifuchun group;State,degeneration and necrosis of mitochondrial structure.The wall cells in the Manpixiao group were secreted,and the mitochondrial structure returned to normal,which was better than the Weifuchun group.3.Results of network pharmacology predictionA total of 17,612 target genes and 723 target genes of PLGC were obtained.The PPI network has 80 nodes and 754 edges,among which the core network module consists of 25 nodes and 254 edges.Enrichment analysis of GO function and KEGG signaling pathway found that the mechanism of PLGC treated by Manpixiao may be related to regulation of intracellular reactive oxygen level,regulation of intracellular oxidative stress state,GO functions such as cell proliferation and apoptosis,as well as regulation of tumor-related signaling pathways and KEGG signaling pathways such as mitochondrial autophagy.4 Ultrastructure of mural cells in each group during PLGC modelingIn the blank model group,the parietal cells were restored,and the mitochondrial structure was denatured and necrotic.The parietal cells in the Manpixiao group were secreted,and the mitochondrial structure was more normal than that in the Weifuchun group.5 Ultrastructure of mural cells in each group during PLGC intervention stageThe parietal cells in the normal group were in a resting state with normal mitochondrial structure was normal,while the parietal cells in the model group were in a secretory state with abnormal and severely damaged mitochondrial structure.Conclusions:1.Manpixiao eliminates significantly the pathological morphology of gastric tissue in PLGC rats,enhances the gastric mucosal barrier function,repairs the pathological state of gastric mucosa atrophy,intestinalization,and dysplasia,and plays a role in preventing and treating PLGC.2,Network pharmacology prediction results,it can be shown that Manpixiao can achieve the effect of treating PLGC by enhancing the level of mitochondrial autophagy in parietal cells and removing damaged mitochondria in parietal cells.3.Manpixiao eliminates the ultrastructure of gastric parietal cells,promotes mitochondrial energy metabolism,regulates the secretion state of parietal cells,enhances the acid secretion function,and delays or blocks the progress of PLGC. |
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