Research On The Analgesic Material Basis And Mechanism Of Sanyu Charcoal

Background:Sanguisorbae radix Carbonisata(SRC)is the product of high temperature carbonization of Sanguisorbae radix,with efficacy of cooling blood to stop bleeding,detoxifying and healing sore.It is clinically used for the treatment of blood syndrome,as well as the pain caused by various diseases such as water and fire burns and carbuncle swollen sores-toxin,which effect is exact.However,there are few studies on the analgesic effect of Sanguisorbae radix Carbonisata,and the research on the basis of the analgesic effect is still a blank.The research on the quality standard of Sanguisorbae radix Carbonisata is slow.The experimental team left behind the old research on small molecular compounds,metal salt ions and other components,creatively introducing the separation technology and characterization methods in the nanomaterials discipline,and comprehensively explored the geothermal charcoal in a new perspective to reveal the material basis and mechanism of action of exerting biological effects.Purposes:1.The Sanguisorbae radix Carbonisata Nano-components(SRC-NCs)are separated from the Sanguisorbae radix Carbonisata.Then the differences of the Sanguisorbae radix Carbonisata Nano-components under different temperature preparation conditions are compared to prefer the best preparation process.2.The Sanguisorbae radix Carbonisata Nano-components are characterized in detail.3.The experimental animal pain models are established to clearly define the analgesic effect of the Sanguisorbae radix Carbonisata Nano-components.4.By measuring the content of inflammatory mediators in serum,the mechanism of analgesic action is initially explored to extend its biological effects.Methods:1.Optimization of preparation process of the Sanguisorbae radix Carbonisata Nano-components: The Sanguisorbae radix Carbonisata is prepared by firing at different gradient temperatures,and the Sanguisorbae radix Carbonisata Nano-components areseparated by water extraction,filteration and dialysis.The comparative analysis of the morphology,physical and chemical characteristics and biological effects of such Nano-components is to optimize the best preparation temperature process,which lays a foundation for the next research.2.Characterization of the Sanguisorbae radix Carbonisata Nano-components: Relying on nanomaterials research techniques,electron microscopy(TEM,HR-TEM),X-ray diffraction analysis(XRD),Fourier transform infrared spectroscopy(FT-IR),Untraviolet-visible absorption spectra(UV-vis)Fluorescence spectroscopy(FL)and X-ray electron spectroscopy(XPS)are used to characterize the Sanguisorbae radix Carbonisata Nano-components in detail.3.Study on the analgesic effect of the Sanguisorbae radix Carbonisata Nano-componentss: Hot plate method and acetic acid writhing method is used to establish the classic animal models of pain,and xylene-induced ear swelling method is used to establish a classic animal model of inflammation,which could evaluate the analgesic effect of the Sanguisorbae radix Carbonisata Nano-components.4.Initial exploration on the analgesic effect and mechanism of the Sanguisorbae radix Carbonisata Nano-components: The degree of analgesia of the drug is evaluated by measuring the content of β-endorphin in the serum.The analgesic mechanism of the Sanguisorbae radix Carbonisata Nano-components are preliminarily determined by measuring the content of inflammatory mediators such as prostaglandin E2 and bradykinin in serum.Results:1.Optimization of preparation process of the Sanguisorbae radix Carbonisata Nano-components: It was found that the morphological appearance and physical and chemical characteristics of the Sanguisorbae radix Carbonisata Nano-components obtained under different temperature preparation conditions have their own characteristics.The biological effect of the Sanguisorbae radix Carbonisata prepared at 350 °C is better.2.Characterization of the Sanguisorbae radix Carbonisata Nano-components: The results of TEM,HR-TEM and XRD analysis showed that the Sanguisorbae radix Carbonisata Nano-components showed an elliptical spherical external morphology with a lattice spacing of 0.243 nm and a uniform distribution.XPS analysis showed that Sanguisorbae radixCarbonisata Nano-components were mainly composed of C,O and a small amount of N element,in which there are multiple chemical bonds such as C-C,C-O,C-N,C=O,C-OH.Combined with FT-IR results,the Sanguisorbae radix Carbonisata Nano-components may contain a variety of reactive groups such as –OH and –COOH.UV-Vis results showed that the Sanguisorbae radix Carbonisata Nano-components have weak UV absorption.3.Study on the analgesic effect of the Sanguisorbae radix Carbonisata Nano-components:The Sanguisorbae radix Carbonisata Nano-components can significantly increase the threshold of pain caused by heat stimulation in mice,reducing the number of writhing caused by chemical stimulation.It can also reduce the rate of ear swelling,which caused by the release of inflammatory mediators induced by proinflammatory reagent,which causes local inflammatory cell infiltration.It is proved that the Sanguisorbae radix Carbonisata Nano-components have good analgesic effect.4.Initial exploration on the analgesic effect and mechanism of the Sanguisorbae radix Carbonisata Nano-components: The Sanguisorbae radix Carbonisata Nano-components can significantly reduce the content of PGE2 and BK in the mice serum and decrease the content of β-EP in it.It is indicated that the analgesic mechanism of the Sanguisorbae radix Carbonisata Nano-components may play a role in improving the inflammatory injury of mice induced by heat and chemical stimulation,thereby reducing the release of inflammatory pain-causing substances such as PGE2 and BK,and down-regulating pain expression of β-EP,by which exerts an analgesic mechanism.Conclusion:In this experiment,the Sanguisorbae radix Carbonisata Nano-components were separated from the the Sanguisorbae radix Carbonisata,and then they were characterized in detail.different analgesic animal models were used to clarify the analgesic effect of the nano-components of the mantle carbon.By measuring the content of various substances in serum,it is found that the analgesic effect may be related to the down-regulation of pain expression of β-endorphin and the release of inflammatory mediators such as prostaglandin E2 and bradykinin.The above conclusions provide new ideas and methods for the research of material basis of Chinese medicine charcoal,and provide experimental basis for the clinical application of the Sanguisorbae radix Carbonisata.