Study On The Repairing Effect And Mechanism Of Shijuemingsan On Ocular Surface Damage In Mice With Dry Eye Model

Purpose:To observe the efficacy of Shi Juming San on C57 / BL6 immunodeficiency mouse dry eye model,and to study the mechanism of Shi Juming San on the treatment of dry eye.study the effect of Shi Jumingsan on C57 / BL6 immunodeficiency mouse model and to understand its possible treatment mechanism.Material and method: Fifty healthy 8-week-old C57 / B6 female mice(purchased from Liaoning Changsheng Biotechnology Co.,Ltd.)were selected,and 35 were selected,randomly divided into 5 groups and divided into blank control groups(N),Model group(M),Shi Jumingsan intragastric group(S),positive drug group(Cs A,solvent group(R),7 rats in each group.Using intelligent dry environment system combined with drug induction,24 C57/ BL6 autoimmune-deficient mice were removed from the blank control group into a dry eye model for two weeks.At the same time,Shijingmingsan was administered to the stomach group(S),positive drug group(Cs A),solvent group(R),and model group(M),respectively,with Shijingming powder decoction,0.05% cyclosporine A drops Eyes,gargle with distilled water for two weeks.On day 0 and day 14,corneal epithelial damage was detected using sodium fluorescein staining method,tear secretion amount was detected by phenol red cotton thread,and tear film rupture time was detected.On day 14,tissues were removed from the eyeballs and eyelid appendages,and frozen pathological sections were performed.Conjunctival goblet cell density was measured by PAS glycogen staining.Corneal epithelial cells and conjunctival epithelial cells were detected by one-step TUNEL method,and corneal and conjunctival CD4 + T cell infiltration was detected by immunofluorescence staining.Results:1.On the 14 th day,the degree of corneal epithelial damage in the model group(M)was significantly different from that of the blank group(P <0.05);the degree of corneal epithelial damage in the Shi Jumingsan gastric irrigation group(S)was significant compared with the model group(M).Difference(P <0.05).2.On the 14 th day,tear secretion in the model group(M)was significantly different from the blank group(P <0.05);tear secretion in the Shi Jumingsan stomach group(S)was significantly different than the model group(M)(P).<0.05).3.On the 14 th day,tear film rupture time in the model group(M)was significantly different from that in the blank group(P <0.05);tear tear time in the Shi Jumingsan gastric gavage group(S)was significant compared with the model group(M).Difference(P <0.05).4.PAS glycogen staining for conjunctival goblet cell density test results showed that the conjunctival goblet cell density of the model group(M)was significantly different from that of the blank group(P <0.05);Shi Jumingsan perfused group(S)The density of goblet cells is significantly different from the model group(M)(P <0.05).5.Using TUNEL one-step method to detect the apoptosis of corneal epithelial cells and conjunctival epithelial cells,the results show that the model group(M)should have a significant difference in the intensity of corneal and conjunctival cells per unit area compared with the blank group(P <0.05);Compared with the model group(M),the fluorescence intensity in the cornea and conjunctiva of the Shijiemingsan gastric gavage group(S)was significantly different from that of the model group(P <0.05).6.CD4 + inflammatory T cell test results by immunofluorescence staining showed that the fluorescence intensity per unit area of CD4 + T cells in the model group(M)was significantly different from that in the blank group(P <0.05);Shi Jumingsan was administered to the stomach The fluorescence intensity per unit area of group(S)was significantly different from that of model group(M)(P <0.05).7.No animal died within 14 days of modeling.Weighing showed that each dose group of the test product had no effect on animal weight gain.(P> 0.05)Conclusion:1.Shi Juming San can alleviate the ocular surface symptoms of the C57 / BL6 dry eye mouse model,and has a repair effect on the ocular surface.2.The possible mechanism that Shi Juming San is effective for dry eye is to protect the conjunctival goblet cells,inhibit the apoptosis of ocular surface cells,and inhibit the ocular surface CD4 + T cells.