"Xing Nao Kai Qiao" Acupuncture Method Combined With Buyang Huanwu Decoction To Study The Spectrum Kinetics Of MCAO Rats

Objectives:(1)to further explain the effect of acupuncture combined with traditional Chinese medicine on cerebral infarction by using cerebral infarction area and nerve function score;(2)spectrum kinetics of acupuncture combined with Chinese medicine alone In comparison,the mechanism of acupuncture combined with synergistic use was clarified from the perspective of changes in the kinetic parameters of Buyang Huanwu Decoction.For the final establishment of acupuncture and the preliminary basic research using spectrum effect kinetics.Methods: Healthy clean grade male SD rats 96(weight 220 to 250g),adaptive feeding for 3 days,pre-mold random numerical table method randomly divided rats into 4 groups: experimental divided into normal group(Group A);Rats,Group A using normal rats,Group B using false surgical rats,only separation of blood vessels,do not carry out hydrant operation,C,D group are using hydrant method to make the rat brain artery embolism(MCAO)model,rats mold sobriety after its nerve function to score,remove the model that has not been successfully molded and the condition is too light.4 groups are normal feeding,free to eat,and give yang also five soup concentrate 0.6ml/ 100 g,1 time a day,continuous gastric for 7 days.B,D group after the rats sober for the first acupuncture treatment,continuous treatment for 7 days,1 time a day.Each group was given blood at the point in time after the 7th day of administration,which was 0,0.083,0.16,0.25,0.5,0.75,1,2,4,8,12,24 h,respectively,with the left eye removed and right Eye removal to take blood,abdominal aorta blood,all take blood 3 ml,blood samples after treatment to detect the blood sample sieos also five soup effective ingredient content.Results:(1)Needle medicine and reduce the Area of MCAO Rat Brain Infarction than the effect due to simple Chinese medicine treatment;(2)wake-up brain tips needle method can promote the absorption anddistribution of Chinese medicine in the body of MCAO rats,slow down the rate of chinese medicine excretion,prolong the retention time of Chinese medicine in the body.