| Purpose:To observe the therapeutic effect by electroacupuncture “Baihui,Yintang,Zusanli” on cerebral ischemia-reperfusion injury(CIRI)rat model,and the influence on the autonomous exercise capacity and the content of changes on neurotrophic factor(BDNF)and receptor(TrkB),which can explore the possible mechanism in term of curing cerebral ischemia-reperfusion injury with electroacupuncture.In order to provide a theoretical basis for better clinical treatment of cerebral ischemia.Material and method:30 healthy adult SD rats,weighing(300 ± 40)g,male,were randomly divided into 2 groups include sham operation group(6 rats)and model replication group(24 rats)Model replication groups were all model-replicated using a modified thread plug method.After the model was replicated,neurological function scores were scored on a scale of 1 to 3 with reference to the 5-grade scale of Zea-Longa et al.[1].The rats were included in the experimental group,and the remaining rats that did not meet the standard were excluded(6 rats died and 5 failed).The 13 rats successfully replicated were randomly divided into 7 rats in the ischemic group(during the experiment,they died 1 Only),electric needle group 6 only.After the treatment,the electroacupuncture group was treated with Baihui,Yintang,and bilateral Zusanli points for therapeutic intervention.Each treatment for 10 minutes was treated once daily for 2 weeks.The ischemic group was only used for modeling without acupuncture treatment.The operation of sham operation group was the same as the ischemia group,except that the thread plug was not inserted.After Electroacupuncture treatment in electroacupuncture group for two weeks continuously,to test the behavioral indexes of rats with open-field experiments,and to score for neurological function.After the end of the experiment,the rats were sacrificed and the ischemic area was excised.Brain tissue around the penumbra region was detected by Western blot to detect the expression of BDNF and TrkB protein in brain tissue of each group.SPSS22.0 software was used for statistical analysis of the experimental results.Results: 1.Effect on nerve function: After modeling,compared with sham operation,the neurological deficit scores of the other two groups were significantly higher than those of the sham operation group,with significant statistical differences(P<0.01),indicating that cerebral ischemia-reperfusion modelling was successful.After treatment,the score in the acupuncture group was lower than that in the ischemia group,which was significantly statistically significant(P<0.01).2.Effect on behavioral signs of rats: The ischemia group's ability of autonomous exercise in the open field experiment was worse than that of the sham operation group(P<0.05);the electroacupuncture group rats' spontaneous exercise ability in the open field experiment was better than the ischemia group(P<0.05).3.Effects of changes in BDNF and TrkB levels: Compared with the sham-operated group,the expression of BDNF and TrkB protein in the penumbra of the ischemic group was significantly increased(P<0.05);Compared with the ischemic group,the expression of BDNF and TrkB protein in the semi-dark brain tissue of acupuncture group was significantly upregulated(P<0.05).Conclusion: 1.Electro-acupuncture at Baihui,Yintang,and Zusanli acupoints can reduce the neurological impairments in rats with cerebral ischemia-reperfusion.2.Electro-acupuncture at Baihui,Yintang and Zusanli can effectively improve the rats' autonomous exercise ability.3.Electro-acupuncture at Baihui,Yintang and Zusanli can promote the brain protection by up-regulating the expression of BDNF and TrkB. |
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