Research On The Integration Of Processing And Processing Of Cork Origin

Purpose:The Phellodendri Chinensis Cortex is the dry bark of the Rutaceae tree with the yellow skin,it tastes bitter,cold in nature.It can clear heat and dampness,detoxicate and treat sores,purging fire and removing steam.The traditional processing method ofPhellodendri Chinensis Cortex can cause a large amount of loss of effective components and reduce the efficacy.Therefore,our research group used processing method of originprocessing integration processing Phellodendri Chinensis Cortex pieces,then compared with the cork pieces processed with the traditional method.According to the content of chemical composition,pharmacological potency of antipyretic and anti-inflammatory to explore the advantages of origin processing integration of Phellodendri Chinensis Cortex,to provideexperimental basis for clinical medication and quality evaluation of Phellodendri Chinensis Cortex pieces.Material and methodMaterial:Phellodendri Chinensis Cortex collected from Ya'an City,Sichuan Province,Yingjing country.Method1.Study on the integration of origin processing and processing ofPhellodendri Chinensis CortexThe shredding width,baking temperature and baking time of Phellodendri Chinensis Cortex as investigation factors,the Berberine hydrochloride,Phellodendrine chloride and dryness degree were used as comprehensive evaluation indexes,using orthogonal design L₉?3⁴?to optimize the best processing technology.2.Study on the integration of origin processing and processing ofPhellodendri Chinensis Cortex processed with saltThe shredding width,frying time,frying temperature and the amount of salt water as investigation factors,the Berberine hydrochloride and Phellodendrine chloride were used as comprehensive evaluation indexes,using orthogonal design L₉?3⁴?to optimize the bestprocessing technology.3.Study on chemical composition of the integration of origin processing and processing of Phellodendri Chinensis CortexDetermining the content of Chlorogenic acid,Phellodendrine chloride,Magnoflorine,Jatrorrhizine hydrochloride,Berberrubine,Palmatine hydrochloride,Berberine hydrochloride,Limonin and Obakunone in origin processing integration and traditional PhellodendriChinensis Cortex by HPLC,then compare them.4.Comparative study on the chemical composition of different partsof the original plant of Phellodendri Chinensis CortexThe chemical composition content of root,root cortex,cortex,leaf and branch of the original plant of Phellodendri Chinensis Cortex were determined by HPLC,and compared them.5.Comparative study on antipyretic effects of heat syndrome rats oforigin processing integration and traditional Phellodendri ChinensisCortexFirstly,rats were divided into 11 groups,then each group of rats were gavageadministration in accordance with the dose of 10mL/kg continuously of 6 days and test rectal temperature,on the 7th day except the blank group,each group of rats subcutaneouslyinjected with 20%dry yeast,gavage administration 0.5 hours after,the blank group gavage the same amount of saline,test rectal temperature every 1 hour,6 hours after modeling,the contents of TNF-?,IL-1?,IL-6,IL-10 and PGE₂ in the hypothalamus of rats were detected,and compare them.6.Comparative study on the effect of origin processing integrationand traditional Phellodendri Chinensis Cortex on acute bacterialperitonitis in ratsFirstly,the rats were divided into 11 groups,then each group of rats were gavageadministration in accordance with the dose of 10 mL/kg continuously of 6 days,on the 7 th days,after 30 minutes of administration,except the blank group,intraperitoneal injection of Escherichia coli suspension and 10%BaSO4 beef soup?1:1?in accordance with the dose of10mL/kg to rats,after 8 hours of modeling,the contents of TNF-?,IL-8?IL-10?CRP?PLA₂in the plasma of rats were detected,and compare them.7.Comparative study on the effect of origin processing integrationand traditional Phellodendri Chinensis Cortex on macrophagesThe origin processing integration and traditional Phellodendri Chinensis Cortex and its processed with salt,we added them with different concentrations in the macrophages cells culture fluid,and then LPS was added after 4 hours,the contents of TNF-?,IL-6,IL-10 and NO in the supernatant were measured after culture of 24 hours,and observed the phagocytosis of macrophages to neutral red.8.Comparative study on the effect of origin processing integrationand traditional Phellodendri Chinensis Cortex on nephrocyteAdding different concentrations of the origin processing integration and traditional Phellodendri Chinensis Cortex and its processed with salt into the nephrocyte cells culturefluid which were stimulated with H₂O₂ for two hours,the contents of BMP-7,Connexin-43,VEGF,PEDF and HGF in the supernatant were measured after culture of 24 hours.9.HPLC Fingerprint Analysis Among Different Processed Products ofPhellodendri Chinensis CortexHPLC was employed to compare fingerprint of ten batches of Phellodendri Chinensis Cortex and its different processed products with mobile phase consisting acetonitril?A?-water?B,containing 0.3%phosphoric acid and 0.3%diethylamine?for gradient elution?0¹0 min,10%¹4%A;10²0 min,14%²5%A;20⁴5 min,25%A?,flow rate is 0.8mL/min,detection wavelength is set at 284 nm,column temperature is 40?and analytic time is 45 min.10.Study on the quality standard of Cortex Phellodendri chinensis andits different processed productsThere are ten batch of Phellodendron chinense that purchasing from different places,we processed them with salt,wine,honey,then we identify them through microscopiccharacteristics and TLC identification.The HPLC was used to determine the content.In addition,the determination of moisture,ash and acid insoluble components and alcohol soluble extracts was carried out.11.Study on processing technology of Phellodendri Chinensis Cortexprocessed with honeyThe contents of the Berberine hydrochloride and Phellodendrine chloride as theevaluation index,adding honey amounts,frying temperature and frying time as factors,using orthogonal design L₉?3⁴?to optimize the best processing technology.12.Study on the difference of oxidative stress in mouse oral fibroblasts before and after Phellodendri Chinensis Cortex processed with honeyUsing 1mmoL/L hydrogen peroxide to establish an oral cellular oxidative damage model,compare the Phellodendri Chinensis Cortex and its processed with honey extracted fromwater and extracted with 70%ethanol and distilled with different polar solvents,after affected the oral cells damaged by oxidation,the difference of SOD activity,NO and MDA content in cells.Results:1.Study on the integration of origin processing and processing ofPhellodendri Chinensis CortexThe best processing technology of the integration of origin processing and processing of Phellodendri Chinensis Cortex is cut width 2⁴ mm,baking temperature 60?and bake6 hours.2.Study on the integration of origin processing and processing ofPhellodendri Chinensis Cortex processed with saltThe best processing technology of the integration of origin processing and processing of Phellodendri Chinensis Cortex processed with salt is cut width 2⁴ mm,frying 5 minutes,frying temperature 150¹60?and the amount of salt water is 2%.3.Study on chemical composition of the integration of origin processing and processing of Phellodendri Chinensis CortexComparing with traditional Phellodendri Chinensis Cortex,the content of chemicalcomposition in the integration of origin processing and processing of Phellodendri Chinensis Cortex is high.4.Comparative study on the chemical composition of different parts of the original plant of Phellodendri Chinensis CortexComparing with the other parts,the chemical composition content in root cortex,cortex of the original plant of Phellodendri Chinensis Cortex is higher.5.Comparative study on antipyretic effects of heat syndrome rats of origin processing integration and traditional Phellodendri Chinensis CortexThe same dose groups compared with the traditional Phellodendri Chinensis Cortex group,the levels of TNF-?,IL-1?,IL-10,IL-6 and PGE₂ were significantly decreased?P<0.05?or extremely significant decreased?P<0.01?in the origin processing integration of Phellodendri Chinensis Cortex,rectal temperature drops.6.Comparative study on the effect of origin processing integration and traditional Phellodendri Chinensis Cortex on acute bacterial peritonitis in ratsThe same dose groups compared with the traditional Phellodendri Chinensis Cortexgroup,the levels of TNF-?,IL-8?IL-10?CRP?PLA₂ were significantly decreased?P<0.05?or extremely significant decreased?P<0.01?in the origin processing integration ofPhellodendri Chinensis Cortex.7.Comparative study on the effect of origin processing integration and traditional Phellodendri Chinensis Cortex on macrophagesThe same dose groups compared with the traditional Phellodendri Chinensis Cortex group,the levels of TNF-?,IL-6,IL-10 and NO were significantly decreased?P<0.05?or extremely significant decreased?P<0.01?in the Phellodendri Chinensis Cortex processed with salt and origin processing integration of Phellodendri Chinensis Cortex and enhanced phagocytosis.8.Comparative study on the effect of origin processing integration and traditional Phellodendri Chinensis Cortex on nephrocyteThe same dose groups compared with the traditional Phellodendri Chinensis Cortex group,the levels of BMP-7,Connexin-43,VEGF and HGF were significantly increased?P<0.05?or extremely significant increased?P<0.01?,the level of PEDF is significantly decreased?P<0.05?or extremely significant decreased?P<0.01?in the Phellodendri Chinensis Cortex processed with salt and origin processing integration of Phellodendri ChinensisCortex.9.HPLC Fingerprint Analysis Among Different Processed Products of Phellodendri Chinensis CortexIts processed products had a similar fingerprint chromatogram to the raw of Phellodendri Chinensis Cortex,but having a little difference in relative content of each component.Some peaks of the HPLC chromatogram were identified as chlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine,berberrabine,palmatinen chloride and berberine hydrochloride etc.Similarities of total mode peaks among ten batches of raw materials,honey,salt and wine processed products were more than 0.9.10.Study on the quality standard of Cortex Phellodendri chinensis and its different processed productsThe Phellodendron powder contains crystal sheath fiber,branched stone cells and square crystal;In the chromatography of the tested products,the same color spots were found in the corresponding positions of the control medicinal materials chromatography and the control drugs chromatography;According to the measured results and requirements in the Chinese Pharmacopoeia 2015 edition:This product is calculated on dry goods,containing berberine hydrochloride(C₂₀H₁₇NO₄·HCl),not less than 3%,containing phellodendrine hydrochloride(C₂₀H₂₃NO₄·HCl),not less than 0.34%,the water shall not exceed 12%,total ash not over8%,acid insoluble components shall not exceed 0.8%;alcohol soluble the extract is not less than 14%.11.Study on processing technology of Phellodendri Chinensis Cortex processed with honeyThe best processing technology of Phellodendri Chinensis Cortex processed with honey:according to the per 100Kg of Phellodendri Chinensis Cortex slices used honey 30Kg,?honey:water=1:1?moistening to honey water completely penetrate into the inside of the medicinal herbs,at 120?to 130?fried4 min.12.Study on the difference of oxidative stress in mouse oral fibroblasts before and after Phellodendri Chinensis Cortex processed with honeyCompared with the blank group,SOD activity and NO content in Phellodendri chinensis Cortex processed with honey group increased significantly?P<0.05?,the content of MDAdecreased significantly?P<0.05?,compared with model group,SOD activity and NO content in Phellodendri chinensis Cortex processed with honey group decreased significantly?P<0.05?,the content of MDA increased significantly?P<0.05?,there were no significant differences in the Phellodendron chinense group.ConclusionThe optimized processing technique of integration of origin processing and processing ofPhellodendri Chinensis Cortex is reasonable and feasible.Comparing with traditional Phellodendri Chinensis Cortex,the content of chemical composition in the integration of origin processing and processing of Phellodendri Chinensis Cortex is high,the quality of the integration oforigin processing and processing of Phellodendri Chinensis Cortex is better.Combined with production practice,the best medicinal part of the original plant of Phellodendri Chinensis Cortex is the cortex.Comparing with traditional Phellodendri Chinensis Cortex,the origin processing integration Phellodendri Chinensis Cortex have more antipyretic effect on heat syndrome rats,the treatment effect is better.The effect of origin processing integration of Phellodendri Chinensis Cortex on acute bacterial peritonitis is stronger and the therapeutic effect is better.From the analysis of the cellular level,comparing with traditional Phellodendri Chinensis Cortex,the integrated processing of Phellodendri Chinensis Cortex cansignificantly reduce the level of inflammatory factors and increase the phagocytic ability of macrophages to neutral red.The origin processing integration of Phellodendri Chinensis Cortex also can repair the injury nephrocyte better.From the fingerprint and quality standard of Phellodendri Chinensis Cortex and its different processed products which were established,we can know that the establishedfingerprint has good precision,stability and reproducibility,which has an important sense of controlling the quality of Phellodendri Chinensis Cortex and its processed products.The method to research on thequality is simple operation,good repeatability,strong stability,which can be applied to the pieces of Phellodendri Chinensis Cortex and its different processed products to control quality.From the research on the processing technology of honeyed Phellodendri Chinensis Cortex,we can know that the best processing technology of Phellodendri Chinensis Cortex processed with honey is reasonable and feasible,it can significantly reduce the oxidativedamage in oral fibroblasts,has a certain therapeutic effect on oral ulcer.