| purpose:Sedum lineare Thunb is a perennial herb of the genus Crassulaceae.It is a commonly used national folk medicine.The purpose of this paper is to systematically separate and identify the chemical constituents of the whole plant,and to study the anti-lung cancer activity.method:1.Separation and purification of total extracts of Sedum lineare Thunb by liquid-liquid extraction,silica gel column chromatography,C18 reverse phase column chromatography,SephadexTM LH-20 gel column chromatography,rapid preparative chromatography and semi-preparative HPLC.The structure of the isolated monomer compound was identified by UV,ESI-MS,NMR and the like.2.CCK-8 method was used to detect the inhibitory effect of total extracts and extracts on the proliferation of non-small cell lung cancer A549 cells.The IC50 value of half-inhibitory concentration was calculated,and the extraction site with better anti-lung cancer activity was selected for monomer.Separation of the compound3.The inhibitory activity of each monomeric compound on non-small cell lung cancer A549 cells was detected by CCK-8 method.The IC50 value of half inhibitory concentration was calculated,and the inhibition of proliferation of A549 cells by each monomeric compound and the positive control drug Ginsenoside Rg3 was compared.effect.4.The effect of each monomeric compound on the transfer of A549 cells was examined by a scratch test.Comparison of effects of monomeric compounds and blank control group on A549 cell metastasisresult:1.8 monomer compounds were isolated and purified from 80%ethanol extract of Sedum lineare Thunb and their structures were identified according to their physicochemical constants and pop analysis.It was identified as Compound 1 Apigenin,Compound 2 3-O-Methylquercetin,Compound 3 Luteolin 4’-O-β-D-(6"-E-caffeoyl)-glucopyranoside,Compound 4 Quercetin,Compound 5 Luteolin,Compound 6 Luteolin-4’-glucoside,compound 7 spiraea,compound 8Adenosine,in which compounds 1,2,3,6,7and8 are the first from the plant Isolated.2.The total extract of Sedum lineare Thunb has a strong inhibitory effect on A549 cells.The ethyl acetate fraction and n-butanol site can strongly inhibit the proliferation of A549 cells.The half inhibition rate is 78.23 μg/mL and 89.45μg/mL,which is very significant compared to the control group.The petroleum ether fraction,methylene chloride fraction and water fraction showed a certain inhibitory activity,but the half inhibitory concentration had exceeded 500 μg/mL,and the inhibitory activity was relatively weak.3 Compound 6 had a certain inhibitory effect on A549 cells,and it was correlated with the dose.With the increase of drug concentration,the inhibition rate increased gradually,and the half inhibitory concentration was 70.47 μmol/L.When the concentration of the compound 4 was higher than 31.25 μmol/L,the inhibition rate was substantially unchanged.When the concentration of compound 5,compound 7,and compound 8 reached 250 μmol/L,the inhibition rate did not reach 50%,and the inhibition rate of these four compounds did not change much with the increase of drug concentration,compared with the positive control Rg3.The inhibition is relatively weak.4.Scratch experiments showed that the six monomeric compounds have a certain inhibitory effect on the metastasis of A549 cells.Among them,the change rate of scratch area of compound 6 was 3.16%,which indicated that compound 6 had a strong inhibitory effect on the transfer of A549 cells.Compared with the blank control group,the rate of change of scratch area of compound 4 and compound 7 was relatively small,and the rate of change of scratch area of compound 8 was large,indicating that compound 4 and compound 7 can strongly inhibit the migration of A549 cells.The role of 8 is relatively weak.Compound 4 and Compound 7 can strongly inhibit the transfer of A549 cells,while Compound 5 and Compound 8 have weaker inhibitory effects.conclusion:8 monomer compounds were isolated and purified from the extracts of Sedum lineare Thunb.They were identified as Compound 1 Apigenin,Compound 2 3-O-Methylquercetin,Compound 3 Luteolin 4’-O-β-D-(6"-E-caffeoyl)-glucopyranoside,Compound 4 Quercetin,Compound 5 Luteolin,Compound 6 Luteolin-4’-glucoside,compound 7 spiraea,compound 8Adenosine,in which compounds 1,2,3,6,7and8 are the first from the plant Isolated.The extracts from the extracts of Sedum lineare Thunb.and the n-butanol sites had strong inhibitory effects on A549 cells.Focus on these two extraction sites.Compound 6 has a certain inhibitory activity on A549 cells,and its inhibition rate is positively correlated with the concentration of administration,while compound 4,compound 5,compound 7,compound 8 have relatively weak anti-lung cancer activity.In this paper,the effects of various parts and chemical constituents of Sedum lineare Thunb on proliferation and metastasis of A549 cells were studied,which provided a basis for further study on the treatment of lung cancer and other tumors. |
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