An Experimental Study On The Effect Of Xiongcanyishen Recipe On Rats With Impotence Due To Liver Depression And Kidney Deficiency

Objective:To investigate the effect of Xiong Can Yi Shen Fang on hair color,body weight,symptoms and reproductive organ quality in rats with liver depression,kidney deficiency and impotence.On tail suspension and mating experiments in rats with liver depression,kidney deficiency and impotence.On Serum T,LH and FSH in Rats with Liver Depression,Kidney Deficiency and Impotence.on the expression of eNOS and cGMP protein in penis of rats with liver depression,kidney deficiency and impotence.Methods: 50 8-week-old SPF male SD rats were selected,and 10 of the 50 male SD rats were selected as normal contra-group rats.The rest rats were all induced with liver depression,kidney deficiency and impotence model for 14 days by intramuscular injection of hydrocortisone injection plus limb restraint.After successful modeling,they were divided into high-dose group(male silkworm high group),dose group(male silkworm middle group),low-dose group(male silkworm low group),Shugan Yiyang capsule group,tadalafil group,model group and blank group according to the random table method,with 5 rats in each group for 28 days.On the 29 th day after drug administration,rats were anesthetized with 10% chloral hydrate(3ml/kg),blood was collected from abdominal aorta,penis tissue of rats was extracted after blood collection,and bilateral testicles(marked left and right sides)were weighed.Suspension tail experiment and mating experiment were used to observe the immobility time of rat suspension tail before and after the model was given.The incubation period(ML),riding times(MF)and insertion times(IF)of rat mating experiment were recorded.Contents of T,LH and FSH in serum were determined by ELISA.Content of eNOS and cGMP protein in penis tissue was determined by immunohistochemical Mean Density analysis method.Results:Compared with the model group,the color and symptoms of male silkworms in high,medium and low dose groups were significantly improved,while those in tadalafil group were not significantly improved.Compared with the model group,there was no significant difference in body weight among the groups(P > 0.05).Compared with the model group,the penis weight of male silkworm high dose group and Shugan Yiyang group increased(P < 0.05).There was no significant difference in the immobility time of tail suspension between the groups before the model was established.After modeling,the fixed time of tail suspension was significantly increased compared with the blank group(P < 0.05).After administration,compared with the model group,the suspended tail immobility time of male silkworm in high,medium and low dose groups and Shugan Yiyang group was significantly reduced(p < 0.05),while there was no significant difference in suspended tail immobility time of tadalafil group(p > 0.05).Compared with the blank group,the ML of the model group was signIFicantly prolonged,MF and if were significantly reduced,with statistically significant difference(p < 0.05).Compared with the model group,the ML of male silkworm in high,medium and low dose groups,Shugan Yiyang group and tadalafil group decreased,while MF and IF increased,the difference was statistically significant(p < 0.05).compared with the blank group,the serum t content in the model group decreased(p < 0.05),while the serum t content in the male silkworm high,medium and low dose groups and the Shugan Yiyang group increased(p < 0.05),while the serum t in tadalafil group did not change significantly(p > 0.05).Compared with the model group,the serum T of male silkworm in high,medium and low dose groups and Shugan Yiyang group increased significantly(P < 0.05).Compared with Shugan Yiyang group,serum T in male silkworm high dose group is higher than Shugan Yiyang group(P < 0.05).Compared with tadalafil group,serum t of male silkworm in high,medium and low dose groups and Shugan Yiyang group increased significantly(p < 0.05).Compared with the model group,there was no significant difference in serum LH levels among the male silkworm high,medium and low dose groups,Shugan Yiyang group and tadalafil group(P > 0.05).Compared with the model group,the high,medium and low dose groups of male silkworm can reduce the serum FSH level(P < 0.05).The positive expression of penis eNOS and cGMP protein in male silkworm groups with high,medium and low dosage,male silkworm group and Shugan Yiyang group was significantly higher than that in model group(p < 0.05),while tadalafil group had no significant difference compared with model group(p > 0.05).The positive expression of eNOS and cGMP protein in penis of male silkworm was significantly higher in high,medium and low dose groups,male silkworm group,Shugan Yiyang group and tadalafil group(P < 0.05).Conclusion:Xiong Can Yi Shen Fang can improve the hair color and symptoms of impotence rats with liver depression and kidney deficiency,but it can not increase the weight of rats,can increase the penis quality,and has no obvious influence on the quality of bilateral testes.Male Silkworm Yishen Recipe can alleviate the depressive symptoms of rats and increase the mating frequency of rats with liver depression,kidney deficiency and impotence.Male Silkworm Yishen Recipe can alleviate the depressive symptoms of rats and increase the mating frequency of rats with liver depression,kidney deficiency and impotence.Male Silkworm Yishen Recipe can increase serum T content and reduce FSH level,which may be one of the mechanisms of male Silkworm Yishen Recipe acting on hypothalamus-pituitary-gonad axis to improve penile erectile function in rats.Male Silkworm Yishen Recipe can activate NO/cGMP pathway and promote the expression of eNOS and cGMP protein in penis tissue.