The Effect Of Low-level Laser Combined With Cerebrospinal Fluid On Neural Differentiation Of Mesenchymal Stem Cells

Low level laser therapy(LLLT),a safe and reliable physical intervention,activates specific photoreceptors after low-energy laser irradiation.Then it could cause photo-thermal effects,photochemical reactions and photosensitive effect,which regulates the immune system,the blood circulation system and the nervous system.On the one hand,LLLT can increase cell proliferation.On the other hand,it can activate the potential for multi-directional differentiation of stem cells at specific wavelengths.Stem cell transplantation has become a new medical technology revolution after drug therapy and surgical treatment.At present,neural differentiation of stem cells induced by biological factors is the safest method,while the induction period is long and the cell activity is greatly weakened.Chemical induction is efficient,but cells are susceptible to chemical toxicity.Based on the current research status,this paper explored the effect of LLLT combined with biological inducers on the regulation of the directional neural differentiation about stem cells from the perspective of cell molecules.The new method could provide theoretical support for the clinical application of LLLT.Human umbilical cord mesenchymal stem cells(hUCMSCs)were separated and cultured in vitro.The results of cell surface antigen included CD31?CD34?CD73?CD90 and CD 105 indicated that the extracted cells were hUCMSCs.The proliferation of hUCMSCs was increased at wavelength of 635 nm quickly,whose the energy density was 3 and 4 J/cm2and the power density was 20 mW/cm2.With LLLT combined with cerebrospinal fluid,the morphology and activity of stem cell proliferation,nerve cell marker protein involved nestin,neuN and GFAP and supernatant secretion contained BDNF,NT-3 and GDNF were detected.The marker protein of neural precursor cell was higher expression at 808 nm combined with inducers after 3 d,which expressed slightly at 635 nm.It indicated that 808 nm was more effective to induce hUCMSCs differentiation.There were great differences between groups about neuN that is marker protein of neuron at 7 d,particularly CSF and 808 nm combined with inducers.However,GFAP had little differences.It concluded that 808 nm combined with CSF/iCSF could increase the ratio of differentiation of hUCMSCs into neurons.The results of supernatant secretion detected by ELISA showed that the expression of NT-3 that is the neuron-specific secretion protein was enhanced gradually in the 808 nm combined with iCSF group,which was better than the single biological inducer.But the concentrations of BDNF and GDNF were lower than the positive groups.Combined effects of low level laser therapy and inducers can effectively promote the differentiation of mesenchymal stem cells into neural precursor cells.808 nm combined with CSF could promote the differentiation of neural precursor cells into neurons and increase the ratio of neuronal differentiation.