The Effect Of PEGDA Gel Modified With Electric Small Molecules On The Proliferation And Differentiation Of Pre-osteoblasts

Objective:To investigate the effect of Sodium Methylallyl Sulfonate(SMAS)modified Poly(ethylene glycol)diacrylate(PEGDA)hydrogel on the proliferation and differentiation of osteoblast-like cells(MC3T3-E1).Methods:A series of negatively charged hydrogels were fabricated by incorporating negatively charged monomer(SMAS)into PEGDA hydrogel at different concentrations and the physical and chemical properties were tested.At days 1,3,5,the proliferation of cells on four hydrogels was assessed by a cell counting kit(CCK-8).At days 7,10,14 of induction,alkaline phosphatase(ALP)activity was investigated for early osteogenic differentiation test.At days 7,14,21 of induction,the osteogenesis related genes expression level of MC3T3-E1 were evaluated by q RT-PCR.At days 21,alizarin red staining was carried out to visualize the calcium content by differentiated cells.Results: SMAS was successfully incorporated into the PEGDA hydrogel,and with increasing concentration of SMAS,the amount of absorbed protein increased in a dose-dependent manner,the proliferation of cells on four hydrogels was significantly enhanced(p < 0.05).Meanwhile,ALP activity was increasing with induction time and the HG200 and HG100 ALP activit was significantly different from that of HG0 group(p < 0.05).The q RT-PCR results revealed enhanced m RNA expression of all markers in cells on four hydrogels,HG100 and HG200 groups were significant different compared with HG0 group(P < 0.05)Conclusion:The SMAS modified hydrogel can effectively promote the cell proliferation and differentiation of MC3T3-E1 cells and would be a promising injectable bone tissue engineering scaffold material.