LSD1 Regulates The Proliferation And Migration Of Non-small Cell Lung Cancer And Its Correlation With PET-CT Imaging

Objective:1.To investigate the relative expression of LSD1(lysine specific demethylase1)in NSCLC(Non-small cell lung cancer)tissues and cells and analyze the association between the expression of LSD1 and clinicopathological information.2.To investigate a series of biological function changes after exogenous LSD1 interference,and analyze the relationship between LSD1 expression and 18F-FDG uptake in NSCLC,evaluating its clinical diagnostic value.Method:1.q RT-PCR(quantitative Real-time PCR)was used to detect the expression of LSD1 in 52 pairs of NSCLC patients tissues and adjacent normal tissues.The relationship between expression of LSD1 and clinical features were analyzed.2.Expression of LSD1 in NSCLC cell A549 and normal lung bronchial epithelial cells16 HBE was detected by q RT-PCR.A549 cells were divided into 2 groups,transfected with LSD1 interfering RNA(si-LSD1 group)and negative control RNA(control group),respectively.Cell proliferation,apoptosis and migration in the two groups were detected by MTT experiment,clone formation experiment,flow cytometry,and transwell migration test.Epithelial-mesenchymal transition related m RNA and protein expression levels were detected by q RT-PCR and Western blotting.3.The subjects were injected 18F-FDG about 185-370MBq(5 to 10 m Ci)by intravenous injection of 4.07MBq/Kg(0.11 m Ci/kg),and the whole body PET-CT imaging was performed.All the images were examined by two experienced nuclear medicine and CT physicians in the PET-CT center.According to the location of the lesion displayed on CT,we draw the same area of interest in the corresponding area of the PET image,and the workstation calculates the SUV of the lesion automatically based on the delineated area of interest.P240 type bronchoscopy(Olympus)was used to puncture the tumor with NA-2C-1 puncture needle(Olympus),parallel biopsy,mucosal needle aspiration cytology and PCR quantitative detection.Result:1.Compared with the adjacent tissues,the expression of LSD1 in NSCLC tissues was over-expressed.The expression of LSD1 was correlated with the TNM stage and lymph node metastasis of NSCLC patients(?2=0.462,P< 0.05),while not correlated with age,sex,smoking,tumor size or pathological typing.2.Compared with the control group,the proliferation and migration ability of A549 cells in si-LSD1 group was decreased,whereas the apoptosis rate was increased.In addition,the expression of E-cadherin was significantly up-regulated(t=5.607,P< 0.05),while vimentin expression was down regulated(t=-6.628,P< 0.05).3.The SUVmax values of early squamous and adenocarcinoma were significantly higher than those in the normal subjects,and significantly lower than those in the middle and late stages.There was no significant difference in SUVmax between early and middle and advanced squamous cell carcinoma and corresponding adenocarcinoma.4.The expression level of LSD1 in advanced squamous cell carcinoma was significantly higher than that in early stage squamous cell carcinoma.The expression level of LSD1 in advanced adenocarcinoma was significantly higher than that in early stage of adenocarcinoma.The expression level of LSD1 was significantly higher in advanced adenocarcinoma.However,there was no significant difference in LSD1 between early and middle and advanced squamous cell carcinoma and corresponding adenocarcinoma.5.There was no significant correlation between the SUVmax and the expression level of LSD1 in early squamous cell carcinoma,while the SUVmax and the expression level of LSD1 in middle and late stage squamous cell carcinoma was significantly correlated.6.The SUVmax and the expression level of LSD1 in early adenocarcinoma was significantly correlated,but there was no significant correlation between the SUVmax and the expression level of LSD1 in middle and late stage adenocarcinoma.Conclusion:The up-regulated expression of LSD1 in NSCLC tissues and cells may be regulated by promoting the involvement of EMT in cell proliferation and migration.The expression level of LSD1 depends on tumor stage and lymph node metastasis.18F-FGD uptake depends on the cell type of non-small cell lung cancer.The correlation between LSD1 and 18F-FGD uptake depends on the stage and cell type of NSCLC.