Analysis Of The Expression Patterns Of CAMR-FT And CAMR-FN In Prostate Tumor Model Mice

Prostate tumor is a common male malignant tumor,mainly occurring in the elderly male population.In recent years,the incidence and mortality rate of prostate cancer in the male population has increased.Therefore,there is an urgent need to find the corresponding targets for the early diagnosis of prostate neoplasms.As an important male reproductive secretory gland,prostate plays an important role in male reproductive health.It is difficult to prevent and cure because of its long incubation period,low early symptoms,high incidence and lack of effective early diagnostic markers.As early as the last century,in order to find the corresponding technical means to solve this problem,the United States,Britain and other Western countries carried out research work on prostate related diseases.Due to the limited medical treatment,China did not attach great importance to prostate related diseases.However,with the gradual increase in China's aging population and prostate cancer mortality,attention has been drawn towards prostate of related diseases.But because the early symptoms are not easy to detect,a lot of the cases are found at the advanced stage of the tumor.Therefore,it is very important to find corresponding proteins for the early detection of prostatic diseases.Recent studies show that there is a certain correlation between the occurrence of prostate tumors and calcium-associated proteins.Calcium ions enter the cells mainly through the calcium channel protein on the cell membrane to maintain the relative homeostasis of intracellular calcium.Calcium homeostasis,such as the increase of calcium influx,increased the concentration of calcium in the cytoplasm and promoted the proliferation of tumor cells.This topic,mainly focus on exploring the expression of CAMR-FT and CAMR-FN proteins in prostatic carcinoma,in order to find tumor markers to provide a theoretical reference for the early diagnosis of prostate cancer.In this study,WTJX91-WTXH985 prostate tumor model mice(tumor mice)were used as the experimental subject,with WTXH985 non tumor mice and Wild Type mice used as control.According to the age and Gleason grade,the tumor mice were divided into early stage(Gleason 2-4),middle stage(Gleason 5-6),and late stage tumors(Gleason 7-10).The histopathological changes of mouse prostate tumor tissue were observed using techniques including Trichrome,HE and PASM staining.Real-time quantity PCR was used to detect the expression of CAMR-FT in tumor mice at different stages;whereas western blot was used to detect the expression of CAMR-FT and CAMR-FN proteins in tumor model mice.Also,immunohistochemistry was used to detect the localization of the expressed proteins of CAMR-FT and CAMR-FN in prostate tumor tissues of mice.Through the implementation of the above topics,the experimental results are as follows:(1)The' results of Real-Time quantitative PCR showed that the expression of CAMR-FT at the diferent stages of prostate tumor was not significant,but the expression level decreased significantly in the advanced stage of the tumor.There was no significant difference in the expression of CAMR-FT in the early stage of tumor compared with the control group,however,the expression of CAMR-FT was significantly up-regulated in the middle stage of tumor compared with the middle-age of 985 mice.Although the expression of CAMR-FT in the late stage of tumor was lower than that in the aged matched 985 mice,there was no significant difference.In the middle-age stage,there was no significant difference between expression of CAMR-FT in tumor and control mice.The expression of CAMR-FT in the advance stage tumor was significantly down regulated compared to the WT counterpart.This suggests that CAMR-FT may play a role in the development of prostate cancer.It is inferred that CAMR-FT may be involved in tumorigenesis and progression.(2)Morphological staining results showed:HE staining of the prostate tumor tissue showed that the glandular structure is irregular,irregular distribution of epithelial cells,tumor morphological difference in different periods is relatively large.Furthermore,PASM staining of prostate tumor tissue showed deep staining of the basement membrane,collagen synthesis increased with increase in both tumor grade and degree of fibrosis.In Masson Trichrome staining analysis,the degree of tumor fibrosis increased obviously with increas in tumor grade.(3)Immunohistochemical analysis showed that in normal prostate tissue in mice,CAMR-FT and CAMR-FN were mainly located in between the basement membrane and epithelial cells in prostate tumors in mice,CAMR-FT distribution between epithelial cells and epithelial cells,between epithelial cells and basement membrane,and along the direction of the expression of epithelial cells decreased;CAMR-FN it is mainly located in the epithelial cells,while epithelial cells also have a small amount of expression.(4)The results of Western Blot showed that compared with the control group,the expression of calcium-associated protein CAMR-FT and CAMR-FN in the prostate tissue of the tumor mice were significantly increased.The expression of CAMR-FT protein was higher in the early stage of tumor,down-regulated at the middle stage,and increased in the late stage.The expression of CAMR-FN protein was higher in the early stage of the tumor,slightly decreased in the middle stage,and increased in the late stage.Conclusion:The above results showed that the regulatory proteins,CAMR-FT and CAMR-FN play a certain role in the occurrence and development of prostate neoplasms.These proteins may be involved in the process of tumor regulation and may have an early warning effect on the occurrence of prostate tumors.Therefore,it can be inferred that CAMR-FT and CAMR-FN may be potential tumor markers for early diagnosis of prostate tumors,which may provide a theoretical basis for the early diagnosis of prostate tumors.