| ObjectiveIn this study,we established sepsis model to observe the effect of rosiglitazone,an agonist of peroxisome proliferator activated receptor(PPAR)on acute lung injury(ALI)in rats with sepsis and to clarify the possible mechanism,so as to provide a theoretical basis for clinical treatment of ALI.MethodsFifty-four healthy male SD rats were randomly divided into three groups:control group(group C),sepsis group(group L),rosiglitazone treatment group(group T).The acute lung injury model of sepsis was established by intraperitioneal injection of 10 mg/kg LPS.The levels of serum TNF-α and IL-6 were measured by enzyme-linked immunosorbent assay(ELISA)at 3h,6h,12h after injection of endotoxin.The lung tissue was stained with hematoxylin and eosin(HE)and observed under light microscopy.The lung wet to dry weight ratio was measured,and the expression of HO-1 protein was detected by western blot.The difference between the two groups was analyzed by one-way ANOVA.The LSD method was used to compare the two groups.Results1.General:L group of rats after intraperitoneal injection of LPS,gradually decreased feeding,decreased activity,increased respiratory rate,apathetic,vertical hair,drowsiness and other symptoms,and with the time going,the above symptoms increased.Compared with L group,T group have obvious improvement.2.Rat lung pathological changes:L group of rats eye:lung volume increases,the surface of congestion,visible grain size of the bleeding point and edema.Light microscopic:visible varying degrees of inflammatory cell infiltration,alveolar collapse,moderate alveolar interval and pulmonary interstitial and alveolar cavity see focal bleeding.Compared with L group,T group at each time point have improved.The wet/dry weight ratio of lung(W/D)L group was significantly higher than that of group C at each time point,and there was significant difference between the two groups at each time point(P<0.01).The lung wet/dry weight of T group was significantly lower than that of L group at 6h and 12h(P<0.01),and the wet/dry weight of group T was gradually increased(P<0.05)at 3h and 6h.4.The serum TNF-α level in the L group were significantly higher than those of group C(P<0.05),and there was significant difference between any two groups at each time point(P<0.05)<0.05),showing a dynamic downward trend.T group 6h,12h was significantly lower than L group(P<0.01);The serum IL-6 level in the L group reached a peak at 3 h,and there was a significant difference(P<0.01)at each time point in the L group at each time point(P<0.01).The levels of serum IL-6 in group T were lower than those in group L(P<0.05).5.Compared with group C,the expression of HO-1 protein in lung tissue of group L increased significantly at 6h and 12h(P<0.01),and the expression of HO-1 protein in L group was significantly higher than that in group L(P<0.01),and there was significant difference between any two groups(P<0.01),and the expression of HO-1 protein in the lung tissue of each group was significantly higher than that of the control group(P<0.01).Showing a dynamic upward trend.ConclusionOur study demonstrated that pretreatment with rosightazone may protect against acute lung injury in septic rats,the mechanism of protective effect may involve in increasing the HO-1 protein in lung tissue and alleviate inflammatory response in sepsis rats. |
