Comparative Analysis Of Non-invasive And Invasive Ventilator In The Treatment Of Chronic Obstructive Pulmonary Disease With Acute Exacerbation-related Respiratory Failure, And Isolation And Air-liquid Interface Culture Of Human And Mouse Primary Airway Ep

Part?The Clinical Research Contrastive Analysis of Noninvasive and Invasive Ventilator Therapy respiratory failure due to Acute Exacerbation of Chronic Obstructive Pulmonary DiseaseBackground: The use of noninvasive mechanical ventilation reduces the mortality of COPD patients with acute exacerbations.This retrospective study was designed for the efficacy and outcome of noninvasive ventilation?NIV?and invasive positive airway pressure?IMV?in the treatment of COPD-induced respiratory failure.Objective: Patients are hospitalized according to NIV or IMV.The clinical parameters of the monitoring include pre-treatment respiratory rate,blood pressure,heart rate and PH,PaCO₂,PaO₂ before and after treatment,concominant diseases such as pulmonary encephalopathy,hypertension,chronic heart failure,diabetes,pneumonia,asthma and so on.Demographic information such as age,sex,length of hospital stay and outcome are recorded.Methods: A total of 40 patients were enrolled in the study.Twenty-seven patients were enrolled in the NIV group and 13 were admitted to the IMV group.Concominant diseases such as pulmonary encephalopathy,hypertension,chronic heart failure,diabetes,pneumonia,asthma and so on are recorded.Demographic information such as age,sex,length of hospital stay and outcome are also recorded.Results: A total of 40 patients were enrolled in the study.Twenty-seven patients were enrolled in the NIV group and 13 were admitted to the IMV group.The average age of the patients in the NIV group was 73.86 years and the average age of the IMV group was 70.75 years?p> 0.5?.The mean values of PH,PaCO₂ and PaO₂ in the NIV group were 7.29 ± 0.94,86.79 ± 27.32 mm Hg,64.39 ± 22.28 mm Hg respectively.The mean value of the above indexes in the IMV group was 7.26 ± 0.13,84.55 ± 26.72 mm Hg and71.25 ± 26.41 mm Hg?p> 0.05?.WBC,PCT in NIV group were 8.23 ± 3.82?×109/L?,0.14 ± 0.21?ng/ml?respectively.IMV group were 15.96 ± 4.88?×109/L?,0.84 ± 0.79?p <0.05?.The mean hospital stay was 10.71 ± 3.609 days in the NIV group and 8.75 ±2.137 days in the IMV group?p> 0.05?.The total number of deaths in the NIV group was 2?5%?,while the number of deaths in the IMV group was 5?38%??p <0.05?.Conclusions: This study suggests that the use of NIV in the treatment of respiratory failure is an effective treatment,with acceptable success rates and lower mortality rates.The use of NIV reduces the rate of tracheal intubation and its complications.Pulmonary infection can aggravate the consciousness of pulmonary encephalopathy,generally choose IMV.Part ? The Basic Research Isolation of Human and Mouse Primary Airway Epithelial Cells and Air-liquid interface CultureObjective: To establish a simple and effective method for primary culture of human and mouse airway epithelial cells,and to construct a model of pseudostratified ciliated columnar epithelium under the condition of air-liquid interface culture.Methods: The human and mouse airway epithelial cells were obtained by tissue patch method and protease XIV low temperature digestion method.Using serum-free epithelial cell culture medium for culture,passaged.The obtained second or third generation cells were identified by morphological and keratin immunofluorescence.And then use the transwell air-liquid interface culture.The construction of pseudostratified ciliated columnar epithelium model was realized and morphological identification was carried out.Results: Tissue block method and protease XIV digestion method were successfully obtained human and mouse airway epithelial cells.The airway epithelial cells obtained by tissue block method and cultrued in serum-free epithelial cell culture medium can be spread to 4 generations.Cell morphology is better,higher purity,more dynamic,but the cell cycle is long,the number of expansion is slower.Low temperature enzyme digestion method to obtain the airway epithelial cell cycle is short,the number of rapid expansion,but the cell is easy to aging and fibroblasts pollution.The keratin immunofluorescence assay was performed on the airway epithelial cells of2-3 generations.The epithelial cells of the two methods reached more than 99% and had high cell purity.Immunofluorescence identification of frozen sections in the transwell medium for 4 weeks was carried out in multiple layers.Conclusions: Tissue block method and low temperature enzyme digestion method are two kinds of simple and effective way to obtain human and mouse airway epithelial cells.The obtained cells have good morphology,high purity and strong activity.Under the condition of air-liquid interface culture,the epithelial cells could form a pseudostratified ciliated columnar epithelium model for 4 weeks,which became the basis of further study on respiratory tract.