Experimental Study Of Curcumin On The Proliferation And Apoptosis Of Renal Cell Carcinoma 786-0 Cell

Objective:To investigate the effect of curcumin on renal cell carcinoma786-0cells HIF-1? and XIAP protein expression level and cell apoptosis, study on the effect of curcumin on renal carcinoma cell growth inhibition and to explore the molecular mechanism of curcumin on renal cell carcinoma, and further reveals the therapeutic effect.Methods:The human renal cell carcinoma786-0cells were divided to5groups as control group, curcumin group (10?moI/L?20?mol/L?40?mo1/L?80?mol/L) The human renal cell carcinoma786-0cells was treated with curcumin of different concentrations for24h,48h and72h respectively.The MTT test was used to evaluate the proliferation inhibition of curcumin on renal cell carcinoma786-0cells and the OD values were computed. The flow cytometry were utilized to observe and detect the apoptosis of renal cell carcinoma786-0cells induced by curcumin.The cell morphological changes of renal cell carcinoma786-0cells were observed by light microscopy after treated with curcumin. The expression of HIF-1? and XIAP protein were evaluated by immunocytochemical detection method.Results:The curcumin of different concentrations could significantly inhibit the proliferation and induce apoptosis of renal cell carcinoma786-0cells,with obvious dose-dependent and time-dependent effects.MTT colorimetry showed that curcumin could inhibit the proliferation of human renal cell carcinoma786-0cell.After treated with different concentrations of curcumin for24h?48h?72h, compared with the control group, the treatment group OD values decreased in varying degrees, there was a statistically significant difference(P<0.05). The comparison between treatment groups also has a statistically significant difference(P<0.05).And with the increasing of concentration of curcumin and prolonging of treatment time, OD value decreased gradually, with increasing concentration of curcumin for24h, proliferation inhibition was increasing, respectively16.89%,40.07%,49.43%,71.29%.with increasing concentration of curcumin for48h, proliferation inhibition was increasing, respectively24.81%,48.69%,64.06%,83.92%.with increasing concentration of curcumin for72h, proliferation inhibition was increasing, respectively25.90%,48.23%,74.05%,88.45%.After the trearment of10umol/L curcumin, proliferation inhibition rate increased with extension of time, After the trearment of10umol/L curcumin, proliferation inhibition rate increased with extension of time, After the trearment of20umol/L curcumin, proliferation inhibition rate increased with extension of time, After the trearment of40umol/L curcumin, proliferation inhibition rate increased with extension of time. After the trearment of80umol/L curcumin, proliferation inhibition rate increased with extension of time.The effect of curcumin on786-0cell proliferation inhibition has obvious dose-effect and time-effect dependent relationship.Flow cytometric detection of apoptosis of 786-0cells showed that after treatment with Oumol/L,10umol/L,20umol/L,40umol/L,80umol/L curcumin for48h, With increasing concentration of curcumin,the effect was dose-effect dependent relationship.Apoptotic rates were:7.27%,19.30%,24.16%,31.05%,86.15%.Compared with the control group, the difference was statistically significant(P<0.05).After20umol/L curcumin treatment,786-0cells had significant morphological changes:The cell density decreased, the cell shrinked and became rounded. With the increasing concentration of curcumin and prolonging of treatment time, the cells gradually detached from the wall of culture bottle. A large number of cells begin to disintegrate and necrosis.Cell cytoplasmic condensation, cytoplasmic and nuclear pyknosis, vacuolus could be observed in the kytoplasm of some cells. Immunocytochemistry results show:With intervention of different concentrations of curcumin for48h,the expression of HIF-la and XIAP protein in renal cell carcinoma786-0cells was decreased as compared with those in the untreated group.Compared with the control group, the difference was statistically significant(P<0.05).Conclusion:1. Curcumin could significantly inhibit the proliferation and induce apoptosis of renal cell carcinoma786-0cells.2. The HIF-1alpha and XIAP protein in human renal cell carcinoma786-0cells have obvious expression.3. Curcumin could down-regulates the human renal cell carcinoma cell line786-0HIF-1? and XIAP protein expression.