| Plant parasitic with wide induce plant diseases cause huge environmental damage and economic losses around the world annually.Biological control of fungi is becoming more and more popular due to nematicides’ risk against environment.Trichoderma is a genus of common filamentous fungi used for biological control,which is widely used as commercial biocontrol reagents in agriculture.One of the most promising biocontrol fungi,the bio-control activity of Trichoderma against plant parasitic has been evaluated in a number of studies.In addition,Species in the Trichoderma genus have the ability to produce a variety of secondary metabolites that play an important role in biological control.They produce a diverse range of SMs that interact with other organisms in various ways,which contribute a lot for its bio-control function.In this study,a putative nonribosomal peptide synthetase(NRPS)NRPS1 was identified in Trichoderma hypoxylon by genome mining approach and the function of this gene’s function was studied by using NRPS1 mutant against the pathogens.Bioinformatics analysis reveals a putative NRPS containing biosynthetic gene cluster that is potential for the biosynthesis of peptaibol.The deletion cassette containing the hygromycin resistance gene was constructed by Quick-change method.The partial gene was deleted by using PEG mediated transformation in T.hypoxylon.NRPS1’s function was identified by the biological activity assay against pathogenic fungi.We identified a putative peptaibol synthesis gene NRPS1 and obtained three deletion mutants.The deletion of NRPS1 significantly decreased the ability to resistance the pathogenic fungi,such as Aspergillus parasiticus,Fusarium oxysporum,and Verticillium alboatrum.NRPS1 is a potential peptaibol synthesis gene in T.hypoxylon and plays important roles in the resistance to pathogenic fungi.This work lays the foundation for the elucidation of peptaibol biosynthesis and its application for the biological control in agriculture. |
