The Effect Of Interaction Between Fusarium And Trichoderma On Cucumber Growth And The Incidence Of Fusarium Wilt

The experiment was conducted in the modern greenhouse and plastic greenhouse of Heilongjiang Bayi Agricultural University from April to August 2018.The cucumber variety"Changchun Mickey"was used as a test material.Potted test method was used to set different levels of Fusarium oxysporum treatment test.The dynamics of Fusarium oxysporum population in the soil and the pathogenesis of cucumber wilt disease were measured and analyzed regularly.And the effect of different levels of Fusarium oxysporum on the growth of cucumber,clear the critical concentration of Fusarium oxysporum;at the same time,the appropriate concentration of Trichoderma 809 conidia and chlamydospores selected from the previous test,and the tip of the critical concentration Fusarium oxysporum was tested by intercropping potted plants.The results of regular detection and analysis of the population dynamics of Trichoderma spp.,Trichoderma spp.,Fusarium oxysporum and the growth of cucumber seedlings were confirmed.The effect of bacteria on the population of conidia and chlamydospores of Trichoderma,the influence of the population of Trichoderma on the population of Fusarium oxysporum,and the dynamics of population changes in the interaction between Trichoderma and Fusarium and the population of the two species Correlation with the incidence of cucumber wilt and cucumber growth,and the ecology of Trichoderma in facility cucumber wilt Governance and the promotion of high-yield production of cucumbers utilization of theoretical basis,to provide technical support for the development and utilization of Trichoderma spp.The research results are as follows:1,10~2,10~3,10~4,10~5,10~6,10~7 cfu/g Fusarium oxysporum spore concentration treatment,the cucumber morphological indicators,material accumulation indicators have a certain inhibitory effect,which 18 days after emergence,when inoculated When the concentration reached 10~4cfu/g,that is,T3,the change trend of each index was obvious.When the inoculum concentration was greater than 10~5 cfu/g,that is,T4,the change of each index tends to be stable.Different concentrations of Fusarium oxysporum had an effect on the physiological indexes and stress resistance indexes of cucumber.At 18 days after emergence,when the concentration of bacteria reached 10~4 cfu/g,that is,T3,the nitrate content increased significantly.When the concentration of inoculum was more than 10~5 cfu/g,that is,T4,the change of nitrate nitrogen content was stable;the soluble sugar content,sucrose content,soluble protein content and other indicators showed a trend of increasing first and then decreasing,18 days after emergence.When the concentration of the inoculation reached 10~4 cfu/g,that is,T3,the indexes changed significantly.When the inoculation concentration was greater than 10~5 cfu/g,that is,T4,the trend of each index was stable;the change of reducing sugar content was not obvious;18d after emergence When the concentration of the inoculated bacteria reaches 10~4 cfu/g,that is,T3,the malondialdehyde content has a significant increase.When the inoculum concentration is greater than 10~5 cfu/g,that is,T4,the malondialdehyde content changes stably;ascorbate peroxidase activity,The activity of superoxide dismutase and polyphenol oxidase activity showed a trend of increasing first and then decreasing.2,10~2,10~3,10~4,10~5,10~6,10~7 cfu/g Fusarium oxysporum spore concentration treatment,the activity of enzymes in the soil and the number of Fusarium wilt pathogens in the soil,the soil invertase increased gradually On the 18th day after emergence,with the increase of Fusarium oxysporum,the activity of invertase decreased gradually;the activity of soil urease increased first and then decreased.At 18 days after emergence,the urease activity of the sterile water control was the highest;soil protease activity It gradually decreased.At 18 days after emergence,the sterile water control,T1,10~2cfu/g Fusarium oxysporum concentration treatment,and T2,10~3cfu/g Fusarium oxysporum,were significantly higher than other treatments;soil phosphate The change trend of enzyme activity is not obvious and has no regularity.With the increase of treatment days,the number of pathogens of Fusarium wilt in soil showed a trend of increasing gradually,and reached the maximum at 18 days after emergence.3,10~2,10~3,10~4,10~5,10~6,10~7 cfu/g Fusarium oxysporum spore concentration treatment,there are different degrees of impact on the occurrence of cucumber wilt disease,with the increase of treatment concentration,the incidence rate and disease index gradually rise high.Correlation analysis showed that the concentration of Fusarium oxysporum in soil was correlated with the incidence rate and disease index.Among them,the correlation between the number of Fusarium oxysporum and the incidence rate in T1 and T2 soils reached a significant level.The correlation between the number of Fusarium oxysporum and the disease index in T2soil reached a very significant level,and other treatments reached significant levels.4,The concentration of 10~4cfu/g of Fusarium oxysporum and Trichoderma harzianum 809concentration of 10~4,10~5cfu/g of chlamydospores and conidia at a concentration of 10~4,10~5cfu/g interaction,cucumber physiological There are certain effects on indicators and rhizosphere soil.On the 22nd day after inoculation,each index showed certain regularity,including peroxidase activity,catalase activity,ascorbate oxidase activity,polyphenol oxidase activity,ascorbate peroxidase activity,superoxide disproportionation.M4 of enzyme activity and other indicators,namely,Fusarium oxysporum and sclerotium spores 10~5cfu/g,were affected by chlamydospores,and the enzyme activity was significantly improved compared with other treatments and controls;malondialdehyde content,plasma membrane For indicators such as permeability and proline,only control 1 inoculated with Fusarium oxysporum 10~4 cfu/g was significantly higher than control 2 treated with other treatments and sterile water.The activities of soil invertase,soil urease and soil protease were not obvious.The soil phosphatase activity showed a gradual decline.At 22 days after inoculation,the control 1 phosphatase activity of Fusarium oxysporum was significantly higher than other treatments.Control 2.The number of pathogens and Trichoderma spores in soil showed a gradual increase trend.The number of spores of Fusarium oxysporum increased slowly.Fusarium oxysporum and sclerotium spores were treated with 10~5cfu/g,ie M4,against spores.Fusarium has the greatest impact,Trichoderma spores have the highest content,and Fusarium oxysporum has the least amount of spores.5,The concentration of 10~4cfu/g of Fusarium oxysporum and Trichoderma harzianum 809concentration of 10~4,10~5 cfu/g of chlamydospores and conidia at a concentration of 10~4,10~5cfu/g,under the conditions of cucumber wilt The occurrence regularity and the control effect have a certain degree of influence.The pathogen 10~4cfu/g interacts with the Trichoderma spores 10~5cfu/g,that is,the M4 disease index is the lowest and the control effect is the best.