| Soybean gray spot disease is a fungal disease that is widespread in many parts of the world and seriously affects the yield and quality of soybean.Breeding gray spot resistance varieties is a more effective method for controlling soybean gray spot.Soybean resistance to gray spot is a quantitative trait.Breeding varieties with molecular markers will shorten the breeding period and improve breeding efficiency.The No.10 physiological race of soybean gray spot disease is one of the dominant species of soybean gray spot in Heilongjiang.Exploring the resistance locus of soybean gray spot 10 is a theoretical basis for breeding resistant varieties.In this study,202 Heilongjiang main soybean varieties were used as experimental materials to construct a natural population.187 pairs of SSR markers were used for genome-wide scanning,and artificial inoculation was used to identify resistance to soybean gray spot disease No.10 physiological races;PowerMarker version 3.25,Structure 2.2 software,Ntsys 2.10 software for population diversity,population structure and distance analysis,and draw adjacent clustering maps with MEGA7.0;using TASSEL2.0 software for linkage disequilibrium,attenuation distance and correlation analysis,When P<0.05,the marker was considered to be significantly associated with the trait,the phenotypic effect value of the allelic variation was calculated,and the excellent allelic variation site and the corresponding carrier material were explored.The main conclusions of this study are as follows:1.202 parts of soybean material resistance to soybean gray spot 10 physiological race showed 15 resistant materials,138 medium resistant materials,48 susceptible materials,the maximum was 66,the minimum was 34,the average The value is 55.62,the coefficient of genetic variation is 0.142,and the population is normally distributed.A total of 808 polymorphic loci were detected,with an average of 4.42 polymorphic loci per marker;the average polymorphism information content index of the population was 0.406;the average gene diversity index(He)was 0.45.The natural population composed of 202 soybean materials can be divided into three sub-populations,which are composed of 90,51,and 61 soybean varieties,and there is a certain degree of genetic confounding among different sub-populations.2.There is a certain degree of linkage disequilibrium between the collinear SSR loci and the non-collinear SSR marker locus.The D’ value in the population decreases with the increase of the genetic distance,and it is found by regression analysis.The attenuation of the D’ value follows the equation y =-0.083 lnx + 0.5817,R2 = 0.2515.7 loci associated with anti-soybean gray spot 10 physiological race were detected,which were satt372 located in D2 linkage group,the contribution rate was 7.14 %;the contribution rate of satt478 on O linkage group 4.01 %;the contribution rate of satt200 on the A1 linkage group was 7.4 %;the contribution rate of satt549 on the N linkage group was 14.74 %;the contribution rate of Satt197 on the B1 linkage group was 9.86 %;The contribution rate of sat-366 was 3.69 %;the contribution rate of satt587 on the I linkage group was 5.36 %.3.Phenotypic effect value and carrier material: The phenotypic effect value analysis of allelic variation of 7 SSR loci associated with resistance to soybean gray spot 10 was found to be against soybean gray spot disease No.10 There were 13 allelic variations of physiological races with synergistic effect.The most common allelic variation was satt587-185,the effect value was 19.58,and the carrier material was ’Dongnong 50’.The lowest allele of the synergistic effect was satt200-249,the effect value was 0.3,and the carrier material was ’Suinong 38’.There were 17 allelic variations with the anti-soybean gray spot 10 physiological race,and the lowest allele was satt549-238 with an effect value of 0.29 and the carrier material was ’Hefeng 22’.,’Heihe 20’.The most significant allelic variation was satt200-278,the effect value was 7.38,and the carrier material was ’Heihe 16’. |
PDF Full Text Request