Study On The Preparation And Performance Of PAN Nanofiber Membrane Modified By Amino Acid Grafting

Hyperbilirubinemia can cause jaundice,hepatitis,and brain damage,and even death in severe cases.The current clinical treament was mainly through hemoperfusion.Bilirubin(BR)adsorbents in hemoperfusion technique contains high adsorption capacity and good blood compatibility.Polyacrylonitrile(PAN)have been used as blood purification material because of their low-cost,easy modification,and membrane forming properties.However,due to the hydrophobic electrospun PAN membranes,it were easy caused membranes fouling,it poor hemocompatibility were not completely thrombosis resistant and need necessary modification prior to their use in biomedical fields.The PAN had high reactive cyanogroup which were easy to chemical grafting modification.In this paper,organic modified saponite(OHec)used as reinforcing phase and polyvinylpyrrolidone(PVP)used as porogen.Electrospun PAN@OHec nanofiber membranes used as matrix.Two schemes were designed to grafted lysine(Lys)onto the surface of PAN@OHec nanofiber membranes.The first scheme was that PAN@OHec nanofiber membranes were modified with amidoxime reaction and cyanogroups were partly converted into amino groups.Lys were gtafted on the membranes by means of the reaction between carboxyl groups and amino groups.The second scheme was that PAN@OHec nanofiber membranes were modified via alkaline hydrolysis,the cyanogroup on the membranes were partially hydrolyzed into carboxyl groups,and then grafted with amino groups of Lys.The morphology,tructure,mechanical properties,wettability,and charging property of the modified Lys-PAN@OHec nanofiber membranes were characterized by Scanning electron microscope(SEM),Fourier transforms infrared spectroscopy(FT-1R),X-ray photoelectron spectroscopy(XPS),Dynamic thermomechanical analysis(DMA),Surface dynamic water contact angle(WCA)and Surface potential(Zeta potential).The anti-pollution properties of nanofiber membranes were investigated by static and dynamic anti-protein adsorption experiments.The blood compatibility were studied by platelet,erythr-ocyte adhesion,and hemolysis test in vitro.For Lys-PANCc(OHec and Lys-HPAN@OHec nanofiber membranes,BR adsorption properties were investigated by temperature,ionic sItrength,and albumin interference.Modified membranes were further studied by the kinetic adsorption and isothermal adsorption.The results showed that the mechanical properties of PAN nanofiber membranes were significantly improved by the addition of OHec.Lys were successfully grafted on the surface of AO-PAN@OHec and HPAN@OHec nanofiber membranes.The hydrophilicity of grafted nanofiber membranes were significantly improved,and modified membranes obtained the good abilit of anti-protein contamination.Lys-PAN@OHec nanofiber membranes exhibited excellent blood compatibility.The maximum adsorption capacities of Lys-PAN@OHec and Lys-HPAN@OHec nanofiber membranes were 35 mg/g and 37 mg/g,and the removal rates were 87%and 93%,respectively.In this paper,we have successfully prepared Lys-PAN@OHec and Lys-HPAN@OHec nanofiber membranes with good BR adsorption properties,which possessed broad application prospects in the fields of blood purification and blood perfusion.